Recombinant Rat TGFB1(Leu30-Arg278) & GARP(Ala18-Leu628) Protein, His-Avi-tagged

• Cat.No.: TGFB1 & GARP-3436R
• Product Overview: Recombinant Rat TGFB1(Leu30-Arg278) & GARP(Ala18-Leu628) Protein(D3ZVD5(GARP)&P17246(TGFB1)) is expressed from HEK293 with His tag and Avi Tag at the C-Terminus.

Specification

• Source: HEK293
• Species: Rat
• Tag: C-His-Avi
• Protein length: Leu30-Arg278 & Ala18-Leu628
• Form: Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Rat GARP&Latent TGF Beta 1 Complex, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human ITGAV&ITGB6, His Tag with the EC50 of 2.0μg/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 69.80 kDa (GARP)&41.28 kDa (Latent TGF beta 1). Due to glycosylation, the protein migrates to 70-80 kDa (GARP)&42-48 kDa&13 kDa (Latent TGF beta 1) based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE
  > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt.
  -80°C for 3-6 months after reconstitution.
  2-8°C for 2-7 days after reconstitution.
  Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Tgfb1 transforming growth factor, beta 1 [ Rattus norvegicus ]
• Official Symbol: TGFB1
• Synonyms: TGFB1; transforming growth factor, beta 1; transforming growth factor beta-1; TGF-beta-1; transforming growth factor, beta-1; Tgfb
• Gene ID: 59086
• mRNA Refseq: NM_021578
• Protein Refseq: NP_067589