Unactive Recombinant Full Length Human AKT1 Protein, N-His-tagged

• Cat.No.: AKT1-17HFL
• Product Overview: N-terminal 6His-tagged recombinant full-length human Akt1/PKB alpha, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA-agarose.

Specification

• Description: Akt1 is an oncogenic AGC kinase that plays a critical role in regulating many processes including cell survival, metabolism, proliferation, growth and angiogenesis. AKT activation relies on the PI3K pathway, and is recognized as a critical node in the pathway. The E17 hotspot is the most characterized of AKT1 mutations, and has been shown to result in activation of the protein. Mutations in AKT1 have also been shown to confer resistance to allosteric kinase inhibitors in vitro. GenBank M63167. The recombinant protein contains the amino acid substitution S478G with respect to this accession number. This conflict is reported in EMBL BE 206796. The residue coordinates in the native sequence are given.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 2mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 50% glycerol, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol.
• Bio-activity: As provided, this lot demonstrated <1% of maximum activity. Activated by phosphorylation with PDK1 and MAPKAP-K2.
• Molecular Mass: 59 kDa
• AA Sequence: 1 MSFFHHHHHH DFDIPTTENL YFQGAMGSMS DVAIVKEGWL HKRGEYIKTW RPRYFLLKND 61 GTFIGYKERP QDVDQREAPL NNFSVAQCQL MKTERPRPNT FIIRCLQWTT VIERTFHVET 121 PEEREEWTTA IQTVADGLKK QEEEEMDFRS GSPSDNSGAE EMEVSLAKPK HRVTMNEFEY 181 LKLLGKGTFG KVILVKEKAT GRYYAMKILK KEVIVAKDEV AHTLTENRVL QNSRHPFLTA 241 LKYSFQTHDR LCFVMEYANG GELFFHLSRE RVFSEDRARF YGAEIVSALD YLHSEKNVVY 301 RDLKLENLML DKDGHIKITD FGLCKEGIKD GATMKTFCGT PEYLAPEVLE DNDYGRAVDW 361 WGLGVVMYEM MCGRLPFYNQ DHEKLFELIL MEEIRFPRTL GPEAKSLLSG LLKKDPKQRL 421 GGGSEDAKEI MQHRFFAGIV WQHVYEKKLS PPFKPQVTSE TDTRYFDEEF TAQMITITPP 481 DQDDSMECVD SERRPHFPQF SYSASGTA
• Purity: Purity 67.7% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: AKT1 AKT serine/threonine kinase 1 [ Homo sapiens (human) ]
• Official Symbol: AKT1
• Synonyms: AKT1; AKT serine/threonine kinase 1; AKT; PKB; RAC; PRKBA; PKB-ALPHA; RAC-ALPHA
• Gene ID: 207
• mRNA Refseq: NM_001014431.2
• Protein Refseq: NP_001014431.1
• MIM: 164730
• UniProt ID: P31749