Active Recombinant Full Length Human NEK2 Protein, N-His-tagged

• Cat.No.: NEK2-115HFL
• Product Overview: N-terminal 6Histagged, recombinant, full-length, human NEK2, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA.

Specification

• Description: NEK (NIMA-Related Kinase) Ser/Thr kinase family includes at least 11 members and is involved in regulation of mitosis. Overexpression of active NEK2 (NLK1) induces a striking splitting of centrosomes, whereas prolonged expression of either active or inactive NEK2 leads to dispersal of centrosomal material and loss of a focused microtubule-nucleating activity.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 0.583mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 1120U/mg, where one unit of NEK2, active activity is defined as 1nmol phosphate incorporated into 0.33mg/ml myelin basic protein per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 52.8 kDa
• AA Sequence: 1 MHHHHHHMPS RAEDYEVLYT IGTGSYGRCQ KIRRKSDGKI LVWKELDYGS MTEAEKQMLV 61 SEVNLLRELK HPNIVRYYDR IIDRTNTTLY IVMEYCEGGD LASVITKGTK ERQYLDEEFV 121 LRVMTQLTLA LKECHRRSDG GHTVLHRDLK PANVFLDGKQ NVKLGDFGLA RILNHDTSFA 181 KTFVGTPYYM SPEQMNRMSY NEKSDIWSLG CLLYELCALM PPFTAFSQKE LAGKIREGKF 241 RRIPYRYSDE LNEIITRMLN LKDYHRPSVE EILENPLIAD LVADEQRRNL ERRGRQLGEP 301 EKSQDSSPVL SELKLKEIQL QERERALKAR EERLEQKEQE LCVRERLAED KLARAENLLK 361 NYSLLKERKF LSLASNPELL NLPSSVIKKK VHFSGESKEN IMRSENSESQ LTSKSKCKDL 421 KKRLHAAQLR AQALSDIEKN YQLKSRQILG MR
• Purity: Purity 65.6% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: NEK2 NIMA related kinase 2 [ Homo sapiens (human) ]
• Official Symbol: NEK2
• Synonyms: NEK2; NIMA related kinase 2; NLK1; RP67; NEK2A; HsPK21; PPP1R111
• Gene ID: 4751
• mRNA Refseq: NM_002497.4
• Protein Refseq: NP_002488.1
• MIM: 604043
• UniProt ID: P51955