| Description : |
NEK3 (NIMA-related kinase 3) is a serine/threonine protein kinase that belongs to the NIMA (Never in Mitosis A)-related kinase family. It is involved in a variety of cellular processes, including cell cycle regulation, mitosis, apoptosis, and cytoskeletal organization. NEK3 has a conserved kinase domain, a C-terminal regulatory domain, and a nuclear localization signal. It is expressed in a wide range of tissues and cell types and is regulated by various post-translational modifications, including phosphorylation, acetylation, and ubiquitination. Recent studies have suggested that NEK3 plays a critical role in promoting tumor growth and survival, making it a potential target for cancer therapy. Additionally, NEK3 has been implicated in a number of other diseases, including heart disease and Alzheimer's disease. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
Full Length |
| Form : |
2.31mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
138U/mg, where one unit of NEK3, active activity is defined as 1nmol phosphate incorporated into 0.33mg/ml myelin basic protein (MBP) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
62.2 kDa |
| AA Sequence : |
1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF KGLRRQMDDY MVLRMIGEGS FGRALLVQHE 61 SSNQMFAMKE IRLPKSFSNT QNSRKEAVLL AKMKHPNIVA FKESFEAEGH LYIVMEYCDG 121 GDLMQKIKQQ KGKLFPEDMI LNWFTQMCLG VNHIHKKRVL HRDIKSKNIF LTQNGKVKLG 181 DFGSARLLSN PMAFACTYVG TPYYVPPEIW ENLPYNNKSD IWSLGCILYE LCTLKHPFQA 241 NSWKNLILKV CQGCISPLPS HYSYELQFLV KQMFKRNPSH RPSATTLLSR GIVARLVQKC 301 LPPEIIMEYG EEVLEEIKNS KHNTPRKKTN PSRIRIALGN EASTVQEEEQ DRKGSHTDLE 361 SINENLVESA LRRVNREEKG NKSVHLRKAS SPNLHRRQWE KNVPNTALTA LENASILTSS 421 LTAEDDRGGS VIKYSKNTTR KQWLKETPDT LLNILKNADL SLAFQTYTIY RPGSEGFLKG 481 PLSEETEASD SVDGGHDSVI LDPERLEPGL DEEDTDFEEE DDNPDWVSEL KKRAGWQGLC 541 DR |
| Purity : |
Purity 84% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
