Active Recombinant Full Length Human NEK4 Protein, C-His-tagged
| Cat.No. : | NEK4-117HFL |
| Product Overview : | C-Terminal 6His-tagged, recombinant, human NEK4, full length, expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography. |
- Specification
- Gene Information
- Related Products
| Description : | NEK4 is a serine/threonine protein kinase required for normal entry into replicative senescence. The encoded protein also is involved in cell cycle arrest in response to double-stranded DNA damage. Finally, this protein plays a role in maintaining cilium integrity, and defects in this gene have been associated with ciliopathies. |
| Source : | Sf21 |
| Species : | Human |
| Tag : | His |
| Protein length : | Full Length |
| Form : | 0.94 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij-35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : | 403 U/mg, where one unit of NEK4 activity is defined as 1 nmol phosphate incorporated into 0.33 mg/ml myelin basic protein (MBP) per minute at 30 centigrade with a final ATP concentration of 100 μM. |
| Molecular Mass : | 96 kDa |
| Purity : | 52% by SDS-PAGE and Coomassie blue staining. |
| Applications : | Drug Discovery & Development |
| Storage : | 1 year at -70 centigrade. |
| Handling Advice : | Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : | On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
| Gene Name : | NEK4 NIMA related kinase 4 [ Homo sapiens (human) ] |
| Official Symbol : | NEK4 |
| Synonyms : | NEK4; NIMA related kinase 4; NRK2; STK2; pp12301 |
| Gene ID : | 6787 |
| mRNA Refseq : | NM_003157.6 |
| Protein Refseq : | NP_003148.2 |
| MIM : | 601959 |
| UniProt ID : | P51957 |
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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