Active Recombinant Full Length Human PI4KA Protein, N-Flag-tagged

• Cat.No.: PI4KA-143HFL
• Product Overview: N-terminal FLAGtagged, recombinant human PI4KIIIα full length. Expressed by baculovirus in Sf21 insect cells. Purified using FLAGTM agarose.

Specification

• Description: PI4KIIIα protein catalyzes the first committed step in the biosynthesis of phosphatidylinositol 4,5-bisphosphate. The mammalian PI 4-kinases have been classified into two types, II and III, based on their molecular mass, and modulation by detergent and adenosine. The protein encoded by this gene is a type III enzyme that is not inhibited by adenosine. Two transcript variants encoding different isoforms have been described for this gene.
• Source: Sf21
• Species: Human
• Tag: Flag
• Protein length: Full Length
• Form: 0.375mg/ml of enzyme in 50mM Tris/HCl pH7.4, 150mM NaCl, 150μg/ml FLAGTM peptide, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 427U/mg, where one unit of PI4KIIIα, active activity is defined as 1nmol phosphatidylinositol 4-phosphate (PI(4)P) formed per minute at room temperature with a final ATP concentration of 100μM.
• Molecular Mass: 233 kDa
• AA Sequence: 1 MGGDYKDDDD KARALCMCPV DFHGIFQLDE RRRDAVIALG IFLIESDLQH KDCVVPYLLR 61 LLKGLPKVYW VEESTARKGR GALPVAESFS FCLVTLLSDV AYRDPSLRDE ILEVLLQVLH 121 VLLGMCQALE IQDKEYLCKY AIPCLIGISR AFGRYSNMEE SLLSKLFPKI PPHSLRVLEE 181 LEGVRRRSFN DFRSILPSNL LTVCQEGTLK RKTSSVSSIS QVSPERGMPP PSSPGGSAFH 241 YFEASCLPDG TALEPEYYFS TISSSFSVSP LFNGVTYKEF NIPLEMLREL LNLVKKIVEE 301 AVLKSLDAIV ASVMEANPSA DLYYTSFSDP LYLTMFKMLR DTLYYMKDLP TSFVKEIHDF 361 VLEQFNTSQG ELQKILHDAD RIHNELSPLK LRCQANAACV DLMVWAVKDE QGAENLCIKL 421 SEKLQSKTSS KVIIAHLPLL ICCLQGLGRL CERFPVVVHS VTPSLRDFLV IPSPVLVKLY 481 KYHSQYHTVA GNDIKISVTN EHSESTLNVM SGKKSQPSMY EQLRDIAIDN ICRCLKAGLT 541 VDPVIVEAFL ASLSNRLYIS QESDKDAHLI PDHTIRALGH IAVALRDTPK VMEPILQILQ 601 QKFCQPPSPL DVLIIDQLGC LVITGNQYIY QEVWNLFQQI SVKASSVVYS ATKDYKDHGY 661 RHCSLAVINA LANIAANIQD EHLVDELLMN LLELFVQLGL EGKRASERAS EKGPALKASS 721 SAGNLGVLIP VIAVLTRRLP PIKEAKPRLQ KLFRDFWLYS VLMGFAVEGS GLWPEEWYEG 781 VCEIATKSPL LTFPSKEPLR SVLQYNSAMK NDTVTPAELS ELRSTIINLL DPPPEVSALI 841 NKLDFAMSTY LLSVYRLEYM RVLRSTDPDR FQVMFCYFED KAIQKDKSGM MQCVIAVADK 901 VFDAFLNMMA DKAKTKENEE ELERHAQFLL VNFNHIHKRI RRVADKYLSG LVDKFPHLLW 961 SGTVLKTMLD ILQTLSLSLS ADIHKDQPYY DIPDAPYRIT VPDTYEARES IVKDFAARCG 1021 MILQEAMKWA PTVTKSHLQE YLNKHQNWVS GLSQHTGLAM ATESILHFAG YNKQNTTLGA 1081 TQLSERPACV KKDYSNFMAS LNLRNRYAGE VYGMIRFSGT TGQMSDLNKM MVQDLHSALD 1141 RSHPQHYTQA MFKLTAMLIS SKDCDPQLLH HLCWGPLRMF NEHGMETALA CWEWLLAGKD 1201 GVEVPFMREM AGAWHMTVEQ KFGLFSAEIK EADPLAASEA SQPKPCPPEV TPHYIWIDFL 1261 VQRFEIAKYC SSDQVEIFSS LLQRSMSLNI GGAKGSMNRH VAAIGPRFKL LTLGLSLLHA 1321 DVVPNATIRN VLREKIYSTA FDYFSCPPKF PTQGEKRLRE DISIMIKFWT AMFSDKKYLT 1381 ASQLVPPDNQ DTRSNLDITV GSRQQATQGW INTYPLSSGM STISKKSGMS KKTNRGSQLH 1441 KYYMKRRTLL LSLLATEIER LITWYNPLSA PELELDQAGE NSVANWRSKY ISLSEKQWKD 1501 NVNLAWSISP YLAVQLPARF KNTEAIGNEV TRLVRLDPGA VSDVPEAIKF LVTWHTIDAD 1561 APELSHVLCW APTDPPTGLS YFSSMYPPHP LTAQYGVKVL RSFPPDAILF YIPQIVQALR 1621 YDKMGYVREY ILWAASKSQL LAHQFIWNMK TNIYLDEEGH QKDPDIGDLL DQLVEEITGS 1681 LSGPAKDFYQ REFDFFNKIT NVSAIIKPYP KGDERKKACL SALSEVKVQP GCYLPSNPEA 1741 IVLDIDYKSG TPMQSAAKAP YLAKFKVKRC GVSELEKEGL RCRSDSEDEC STQEADGQKI 1801 SWQAAIFKVG DDCRQDMLAL QIIDLFKNIF QLVGLDLFVF PYRVVATAPG CGVIECIPDC 1861 TSRDQLGRQT DFGMYDYFTR QYGDESTLAF QQARYNFIRS MAAYSLLLFL LQIKDRHNGN 1921 IMLDKKGHII HIDFGFMFES SPGGNLGWEP DIKLTDEMVM IMGGKMEATP FKWFMEMCVR 1981 GYLAVRPYMD AVVSLVTLML DTGLPCFRGQ TIKLLKHRFS PNMTEREAAN FIMKVIQSCF 2041 LSNRSRTYDM IQYYQNDIPY
• Purity: Purity 65% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PI4KA phosphatidylinositol 4-kinase alpha [ Homo sapiens (human) ]
• Official Symbol: PI4KA
• Synonyms: PI4KA; phosphatidylinositol 4-kinase alpha; SPG84; GIDID2; PIK4CA; PMGYCHA; pi4K230; PI4K-ALPHA
• Gene ID: 5297
• mRNA Refseq: NM_058004.4
• Protein Refseq: NP_477352.3
• MIM: 600286
• UniProt ID: P42356