Active Recombinant Full Length Human STK33 Protein, N-His-tagged

• Cat.No.: STK33-173HFL
• Product Overview: N-terminal 6Histagged, recombinant, full length, human STK33, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: STK33 (Serine/Threonine Kinase 33) is predicted to be involved in mitotic DNA damage checkpoint signaling and protein autophosphorylation. It also predicted to be located in perinuclear region of cytoplasm. It may play a specific role in the dynamic behavior of the intermediate filament cytoskeleton by phosphorylation of VIME (by similarity).
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 1.621mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 33U/mg, where one unit of STK33 activity is defined as 1nmol phosphate incorporated into 0.33mg/ml myelin basic protein per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 62.4 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF KGLRRRMADS GLDKKSTKCP DCSSASQKDV 61 LCVCSSKTRV PPVLVVEMSQ TSSIGSAESL ISLERKKEKN INRDITSRKD LPSRTSNVER 121 KASQQQWGRG NFTEGKVPHI RIENGAAIEE IYTFGRILGK GSFGIVIEAT DKETETKWAI 181 KKVNKEKAGS SAVKLLEREV NILKSVKHEH IIHLEQVFET PKKMYLVMEL CEDGELKEIL 241 DRKGHFSENE TRWIIQSLAS AIAYLHNNDI VHRDLKLENI MVKSSLIDDN NEINLNIKVT 301 DFGLAVKKQS RSEAMLQATC GTPIYMAPEV ISAHDYSQQC DIWSIGVVMY MLLRGEPPFL 361 ASSEEKLFEL IRKGELHFEN AVWNSISDCA KSVLKQLMKV DPAHRITAKE LLDNQWLTGN 421 KLSSVRPTNV LEMMKEWKNN PESVEENTTE EKNKPSTEEK LKSYQPWGNV PDANYTSDEE 481 EEKQSTAYEK QFPATSKDNF DMCSSSFTSS KLLPAEIKGE MEKTPVTPSQ GTATKYPAKS 541 GALSRTKKKL
• Purity: Purity = 87.4% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: STK33 serine/threonine kinase 33 [ Homo sapiens (human) ]
• Official Symbol: STK33
• Synonyms: STK33; serine/threonine kinase 33
• Gene ID: 65975
• mRNA Refseq: NM_001289058.2
• Protein Refseq: NP_001275987.1
• MIM: 607670
• UniProt ID: Q9BYT3