Active Recombinant Human BMPR1B(148-end) Protein, N-His-tagged

• Cat.No.: BMPR1B-21H
• Product Overview: N-terminal 6Histagged, recombinant, human ALK6 amino acids 148-end expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: ALK6 is a member of the bone morphogenetic protein (BMP) receptor family of transmembrane serine/threonine kinases. The ligands of this receptor are BMPs, which are members of the TGF-beta superfamily. BMPs are involved in endochondral bone formation and embryogenesis. These proteins transduce their signals through the formation of heteromeric complexes of 2 different types of serine (threonine) kinase receptors: type I receptors of about 50-55 kD and type II receptors of about 70-80 kD. Type II receptors bind ligands in the absence of type I receptors, but they require their respective type I receptors for signaling, whereas type I receptors require their respective type II receptors for ligand binding. Mutations in this gene have been associated with primary pulmonary hypertension. Several transcript variants encoding two different isoforms have been found for this gene.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 148-end
• Form: 0.531mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 159U/mg, where one unit of ALK6, active activity is defined as 1nmol phosphate incorporated into casein per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 44 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF RYKRQETRPR YSIGLEQDET YIPPGESLRD 61 LIEQSQSSGS GSGLPLLVQR TIAKQIQMVK QIGKGRYGEV WMGKWRGEKV AVKVFFTTEE 121 ASWFRETEIY QTVLMRHENI LGFIAADIKG TGSWTQLYLI TDYHENGSLY DYLKSTTLDA 181 KSMLKLAYSS VSGLCHLHTE IFSTQGKPAI AHRDLKSKNI LVKKNGTCCI ADLGLAVKFI 241 SDTNEVDIPP NTRVGTKRYM PPEVLDESLN RNHFQSYIMA DMYSFGLILW EVARRCVSGG 301 IVEEYQLPYH DLVPSDPSYE DMREIVCIKK LRPSFPNRWS SDECLRQMGK LMTECWAHNP 361 ASRLTALRVK KTLAKMSESQ DIKL
• Purity: Purity 89% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: BMPR1B bone morphogenetic protein receptor type 1B [ Homo sapiens (human) ]
• Official Symbol: BMPR1B
• Synonyms: BMPR1B; bone morphogenetic protein receptor type 1B; ALK6; AMD3; AMDD; BDA2; ALK-6; BDA1D; CDw293
• Gene ID: 658
• mRNA Refseq: NM_001203.3
• Protein Refseq: NP_001194.1
• MIM: 603248
• UniProt ID: O00238