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Recombinant Human BMPR1B cell lysate

Cat.No. : BMPR1B-464HCL
Product Overview : Human BMPR1B / ALK6 (149-502) derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls
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  • Gene Information
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Source : Baculovirus-Insect Cells
Species : Human
Preparation method : Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
Lysis buffer : Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Quality control Testing : 12.5% SDS-PAGE Stained with Coomassie Blue
Recommended Usage : 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
Stability : Samples are stable for up to twelve months from date of receipt at -80°C
Storage Buffer : 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
Storage Instruction : Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
Gene Name : BMPR1B bone morphogenetic protein receptor, type IB [ Homo sapiens ]
Official Symbol : BMPR1B
Synonyms : BMPR1B; bone morphogenetic protein receptor, type IB; bone morphogenetic protein receptor type-1B; ALK6; CDw293; BMPR-1B; BMP type-1B receptor; activin receptor-like kinase 6; serine/threonine receptor kinase; ALK-6;
Gene ID : 658
mRNA Refseq : NM_001203
Protein Refseq : NP_001194
MIM : 603248
UniProt ID : O00238
Chromosome Location : 4q23-q24
Pathway : Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Ovarian Infertility Genes, organism-specific biosystem; Signal Transduction, organism-specific biosystem; Signaling by BMP, organism-specific biosystem; TGF-beta signaling pathway, organism-specific biosystem; TGF-beta signaling pathway, conserved biosystem;
Function : ATP binding; SMAD binding; activin receptor activity, type II; metal ion binding; nucleotide binding; protein binding; protein serine/threonine kinase activity; receptor activity; receptor signaling protein serine/threonine kinase activity; transforming growth factor beta-activated receptor activity; transmembrane receptor protein serine/threonine kinase activity; transmembrane receptor protein serine/threonine kinase activity; transmembrane receptor protein serine/threonine kinase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (5)

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Can BMPR1B be targeted for regenerative medicine purposes? 02/19/2023

Yes, the role of BMPR1B in tissue regeneration, especially in bone and cartilage, makes it a potential target for regenerative medicine approaches.

In what ways can BMPR1B be targeted for therapeutic purposes? 07/13/2017

Therapeutic strategies may involve the use of small molecules, antibodies, or gene therapies to modulate BMPR1B activity for treating various clinical conditions.

How does BMPR1B protein contribute to fertility in humans? 11/06/2016

BMPR1B is involved in the regulation of ovarian function and follicular development, making it relevant to fertility treatments and reproductive medicine.

How does BMPR1B contribute to the development of the nervous system? 07/25/2016

BMPR1B is involved in neural development, and understanding its role could have implications for neuroregenerative medicine and the treatment of neurodevelopmental disorders.

Is BMPR1B associated with any autoimmune diseases? 02/04/2016

While the direct association with autoimmune diseases is not well-established, the role of BMPR1B in immune regulation might be explored for potential implications in autoimmune disorders.

Customer Reviews (3)

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Reviews
07/24/2021

    Great performance in ELISA.

    02/02/2018

      Researchers can confidently rely on this high-quality protein to produce accurate and reproducible results, thereby accelerating their research progress

      08/15/2017

        Its well-established reputation and reliability further contribute to its suitability for a wide range of experimental designs.

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