| Description : |
Fibroblast growth factor (FGF) superfamily consists of 23 members, all of which contain a conserved 120 amino acid (aa) core region that contains six identical, interspersed amino acids. Based on the pI factor, two main groups of FGF are named: acidic FGF (aFGF) and basic FGF (bFGF). The superfamily members act extracellularly through four tyrosine kinase FGF receptors (FGFR1 through FGFR4), with multiple specificities noted for almost all FGFs. Biological binding of aFGF to its receptors will result in phosphorylation of the receptor and non-receptor proteins and generate its function. Overexpression and amplification of the fibroblast growth factor receptor-1 (FGFR-1), a prototypic receptor tyrosine kinase, have been found in tumor samples from human pancreas, breast, brain, prostate, thyroid gland, and salivary gland. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
GST |
| Protein length : |
456-765 aa |
| Form : |
2.008mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
1861U/mg, where one unit of FGFR1 activity is defined as 1nmol phosphate incorporated into 250μM (KKKSPGEYVNIEFG) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
62 kDa |
| AA Sequence : |
1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPEF MLAGVSEYEL 241 PEDPRWELPR DRLVLGKPLG EGCFGQVVLA EAIGLDKDKP NRVTKVAVKM LKSDATEKDL 301 SDLISEMEMM KMIGKHKNII NLLGACTQDG PLYVIVEYAS KGNLREYLQA RRPPGLEYCY 361 NPSHNPEEQL SSKDLVSCAY QVARGMEYLA SKKCIHRDLA ARNVLVTEDN VMKIADFGLA 421 RDIHHIDYYK KTTNGRLPVK WMAPEALFDR IYTHQSDVWS FGVLLWEIFT LGGSPYPGVP 481 VEELFKLLKE GHRMDKPSNC TNELYMMMRD CWHAVPSQRP TFKQLVEDLD RIVALTSNQE |
| Purity : |
Purity 75.1% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
