| Description : |
Two high affinity VEGF receptors, FLT1 (VEGFR-1, activated by placental growth factor PlGF) and KDR (Flk-1/VEGFR-2, activated by VEGF), have been identified. These receptors can be divided into three structural regions: seven extracellular Ig-like domains that contain the growth factor binding sites, a single polypeptide chain hydrophobic trans-membrane sequence, and intracellular cytoplasmic domains that confer the tyrosine kinase activity required for signal transduction. VEGF-B and VEGF-D homologues bind with high affinity to FLT1 (fms-related tyrosine kinase 1, vascular endothelial growth factor/vascular permeability factor receptor). |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
GST |
| Protein length : |
783-end |
| Form : |
0.502mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
344U/mg, where one unit of Flt1, active activity is defined as 1nmol phosphate incorporated into 250μM (KKKSPGEYVNIEFG) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
89 kDa |
| AA Sequence : |
1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSMKRS SSEIKTDYLS 241 IIMDPDEVPL DEQCERLPYD ASKWEFARER LKLGKSLGRG AFGKVVQASA FGIKKSPTCR 301 TVAVKMLKEG ATASEYKALM TELKILTHIG HHLNVVNLLG ACTKQGGPLM VIVEYCKYGN 361 LSNYLKSKRD LFFLNKDAAL HMEPKKEKME PGLEQGKKPR LDSVTSSESF ASSGFQEDKS 421 LSDVEEEEDS DGFYKEPITM EDLISYSFQV ARGMEFLSSR KCIHRDLAAR NILLSENNVV 481 KICDFGLARD IYKNPDYVRK GDTRLPLKWM APESIFDKIY STKSDVWSYG VLLWEIFSLG 541 GSPYPGVQMD EDFCSRLREG MRMRAPEYST PEIYQIMLDC WHRDPKERPR FAELVEKLGD 601 LLQANVQQDG KDYIPINAIL TGNSGFTYST PAFSEDFFKE SISAPKFNSG SSDDVRYVNA 661 FKFMSLERIK TFEELLPNAT SMFDDYQGDS STLLASPMLK RFTWTDSKPK ASLKIDLRVT 721 SKSKESGLSD VSRPSFCHSS CGHVSEGKRR FTYDHAELER KIACCSPPPD YNSVVLYSTP 781 PI |
| Purity : |
Purity 74% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
