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Flt3l

  • Official Full Name

    FMS-like tyrosine kinase 3 ligand

  • Overview

    FLT3L, also known as flt3 ligand, acts as a growth factor that increases the number of immune cells by activating the hematopoietic progenitors. It is a small molecule which also induces the mobilization of the hematopoietic progenitors and stem cells in
  • Synonyms

    Flt3l; FMS-like tyrosine kinase 3 ligand; Ly72L; Flt3lg; fms-related tyrosine kinase 3 ligand; flt3 ligand;

  • Recombinant Proteins
  • GMP Proteins
  • Cell & Tissue Lysates
  • Protein Pre-coupled Magnetic Beads
  • Human
  • Macaca mulatta
  • Mouse
  • Barley Grain
  • CHO
  • CHO-K1
  • E.coli
  • HEK293
  • HEK293T
  • Human Cell
  • Insect Cell
  • Mammalian cells
  • C
  • hFc
  • His
  • Fc
  • His (Fc)
  • Avi
  • His|T7
  • Myc
  • DDK
  • N/A
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human FLT3L-20H Active Recombinant Human FLT3L, His-tagged Barley Grain His
Human Flt3L-01HG Active GMP Recombinant Human Flt3L Protein CHO-K1
Human FLT3L-1888H Active Recombinant Human FLT3L protein HEK293 N/A Thr 27 - Pro 185
Mouse Flt3l-03M Active Recombinant Mouse Flt3l E.coli N/A
Mouse Flt3l-936M Active Recombinant Mouse Flt3l protein E.coli N/A 163 amino acids
Mouse Flt3l-01M Active Recombinant Mouse Flt3l Protein, His-Tagged E.coli His
Mouse Flt3l-188M Recombinant Mouse FMS-like Tyrosine Kinase 3 Ligand Mammalian cells N/A
Mouse Flt3l-8704M Recombinant Mouse Flt3l, Fc tagged Human Cell Fc
Mouse Flt3l-457M Recombinant Mouse Flt3l protein, His-tagged HEK293 His Gly27-Arg188
Mouse Flt3l-456M Active Recombinant Mouse Flt3l protein(Met1-Arg188), hFc-tagged HEK293 C-hFc Met1-Arg188
Mouse Flt3l-762M Recombinant Mouse Flt3l protein, His & T7-tagged E.coli His/T7 Gly27~Gln189
Mouse Flt3l-63M Recombinant Mouse Flt3l protein, His-tagged Insect Cell His
Mouse FLT3LG-1387MCL Recombinant Mouse FLT3LG cell lysate Human Cell N/A
Mouse Flt3l-3046M Recombinant Mouse Flt3l Protein, Myc/DDK-tagged HEK293T Myc/DDK
Mouse Flt3l-4372M Active Recombinant Mouse Flt3l Protein E.coli N/A
Mouse Flt3l-02M Active Recombinant Mouse Flt3l Protein, His-tagged CHO His
Mouse Flt3l-064M Active Recombinant Mouse Flt3l Protein E.coli
Mouse Flt3l-10556M Recombinant Mouse Flt3l Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Mouse Flt3l-10556M-B Recombinant Mouse Flt3l Protein Pre-coupled Magnetic Beads HEK293
Mouse Flt3l-26M Active Recombinant Mouse Flt3l Protein (Gly27-Arg188), C-His tagged, Animal-free, Carrier-free E.coli C-His Gly27-Arg188
Mouse FLT3L-99M Active Recombinant Mouse FLT3L Protein E.coli
Mouse Flt3l-285F Active Recombinant Mouse Flt3l Protein, His-tagged CHO His
Macaca mulatta FLT3-1229M Active Recombinant Macaca mulatta FLT3 Ligand E.coli N/A 159aa
  • Background
  • Quality Guarantee
  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • Other Resource

What is flt3l protein?

FLT3L is a disulfide-linked homodimer, with each monomer composed of 308 amino acids. It belongs to the platelet-derived growth factor/vascular endothelial growth factor family of cytokines. Binds to and signals through FLT3 (Fms-like tyrosine kinase 3) receptor expressed on hematopoietic stem/progenitor cells. Produced by stromal cells, dendritic cells, endothelial cells in bone marrow and lymph nodes.

What is the function of flt3l protein?

Hematopoiesis regulation: Stimulates the proliferation and differentiation of hematopoietic stem cells and progenitors of myeloid and lymphoid lineages.

Dendritic cell development: Plays a critical role in the differentiation and survival of dendritic cell progenitors in the bone marrow and periphery.

B cell production: Promotes the development and expansion of B cell progenitors in the bone marrow.

Natural killer cell development: Involved in the differentiation and maintenance of natural killer cell lineages.

Flt3l related signaling pathway

JAK-STAT pathway: Binding of FLT3L to FLT3 induces phosphorylation and activation of Janus kinases (JAKs) which then activate STAT proteins.

Ras-Raf-MEK-ERK pathway: FLT3L stimulation leads to activation of the Ras GTPase and downstream Raf, MEK and ERK kinases.

PI3K-Akt pathway: FLT3L receptor ligation activates the phosphoinositide 3-kinase (PI3K) which triggers the Akt signaling cascade.

FLT3L Related Diseases

Acute myeloid leukemia (AML): FLT3 receptor mutations seen in around 30% of AML cases, linked to poorer prognosis.

Immunodeficiencies: Deficiencies in FLT3L or its receptor FLT3 can impair dendritic cell development and function.

Autoimmune diseases: FLT3 hyperactivity has been implicated in autoimmune conditions like rheumatoid arthritis.

Hematopoietic stem cell mobilization failure: Low FLT3L levels impede stem cell recruitment into peripheral blood circulation.

Biomedical Application of FLT3L Protein

  •  Cancer immunotherapy: Used as an adjuvant to boost dendritic cell responses against tumors.
  •  Bone marrow transplantation: Help promote stem cell engraftment after hematopoietic stem cell transplantation.
  •  Wound healing: May accelerate healing through mobilization of progenitor cells to injury sites.
  •  Dendritic cell vaccines: Added to cultures to generate ex vivo dendritic cells for anticancer/antiviral vaccines.

High Purity

SDS-PAGE Flt3l-01M.jpg

Fig1. SDS-PAGE (Cat. No.: Flt3l-01M)

High Bioactivity & Detection Sensitivity

Flt3l bioactivity.jpg

Fig2. The protein has a calculated MW of 19.9 kDa. The protein migrates as 22-28 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Serum Flt3L at day 26 in patients.jpg

(Paul Milne, 2019)

Fig3. Serum Flt3L at day 26 in patients who subsequently achieved or did not achieve CR. Dotted line indicates optimal cut point identified by the ROC curve: 116 pg/mL (Mann-Whitney UP value).

Case 2: Clappaert EJ, Kancheva D, Brughmans J, et al. Flt3L therapy increases the abundance of Treg-promoting CCR7+ cDCs in preclinical cancer models. Front Immunol. 2023 Aug 9;14:1166180. doi: 10.3389/fimmu.2023.1166180. PMID: 37622122; PMCID: PMC10445485.
Flt3L is a cytokine required for DC development that can increase DC abundance in the tumor when administered therapeutically. However, the impact of Flt3L on the phenotype of distinct cDC subsets in the tumor microenvironment is still largely undetermined. Here, using multi-omic single-cell analysis, researchers show that Flt3L therapy increases all cDC subsets in orthotopic E0771 and TS/A breast cancer and LLC lung cancer models, but this did not result in a reduction of tumor growth in any of the models.

Flt3L therapy does not improve the αCD40-mediated tumor-growth reduction.jpg

Fig4. Flt3L therapy does not improve the αCD40-mediated tumor-growth reduction. (D) E0771 tumor growth upon Flt3L+αCD40 therapy.

Flt3l involved in several pathways and played different roles in them. We selected most pathways Flt3l participated on our site, such as Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Pathways in cancer, which may be useful for your reference. Also, other proteins which involved in the same pathway with Flt3l were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
Cytokine-cytokine receptor interactionCLCF1;Ifna15;CCL20B;BMP7;CCL17;CCR8;TNFSF12;IL12B;OSM
Hematopoietic cell lineageIL11;FLT3LG;ITGB3;IL3;CD34;CD8B1;IL1R1;IL3RA;CD59
Pathways in cancerNCOA4;GSTP1;GNGT2;FZD6;MECOM;BMP2;FGF8;CKS2;PRKCA

Flt3l has several biochemical functions, for example, cytokine activity, protein homodimerization activity, receptor tyrosine kinase binding. Some of the functions are cooperated with other proteins, some of the functions could acted by Flt3l itself. We selected most functions Flt3l had, and list some proteins which have the same functions with Flt3l. You can find most of the proteins on our site.

Function Related Protein
cytokine activityCD70;CCL20B;BMP8B;AIMP1;OSTN;IFNT2;WNT7A;Il1f9;IFNA14
protein homodimerization activityBOK;DSCAML1;PDGFB;ZBTB1;CHKA;NKX2-5;LRRK2;ENG;HSP90AA1
receptor tyrosine kinase bindingANGPT2A;NCK1;PTPN14;NRG3;TP53;PLCG1;DOCK4;SQSTM1;TOB1

Flt3l has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with Flt3l here. Most of them are supplied by our site. Hope this information will be useful for your research of Flt3l.

Research Area

Related articles

Ramos, MIP; Karpus, ON; et al. Absence of Fms-like tyrosine kinase 3 ligand (Flt3L) signalling protects against collagen-induced arthritis. ANNALS OF THE RHEUMATIC DISEASES 74:211-219(2015).
Hodge, DL; Berthet, C; et al. IFN-gamma AU-rich element removal promotes chronic IFN-gamma expression and autoimmunity in mice. JOURNAL OF AUTOIMMUNITY 53:33-45(2014).
  • Q&As
  • Reviews

Q&As (5)

Ask a question
In what ways does FLT3L impact the differentiation of hematopoietic progenitor cells? 02/21/2023

FLT3L promotes the differentiation of hematopoietic progenitor cells into various blood cell lineages, contributing to the diversity of blood cell types.

Are there any side effects associated with FLT3L-based therapies? 10/07/2022

Common side effects may include flu-like symptoms, fatigue, and gastrointestinal disturbances, but the severity varies among individuals.

How does FLT3L influence dendritic cell development and function? 06/13/2022

FLT3L is a crucial factor in the development and maturation of dendritic cells, playing a role in immune response regulation.

How does FLT3L contribute to the maintenance of hematopoietic stem cells in the bone marrow? 06/30/2021

FLT3L supports the maintenance and self-renewal of hematopoietic stem cells in the bone marrow, influencing the overall balance of blood cell production.

Can FLT3L be used as a prognostic marker in leukemia patients? 05/22/2017

Yes, elevated FLT3L levels can serve as a prognostic marker, helping to predict the likelihood of relapse and overall survival in leukemia patients.

Customer Reviews (3)

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Reviews
01/23/2023

    I am confident in the manufacturer's ability to deliver outstanding technical support.

    04/04/2021

      By incorporating the FLT3L protein into my experiments, I am confident that I am utilizing a product that meets the highest standards of quality and performance.

      08/28/2018

        Its high purity and integrity provide the necessary assurance for consistent and reliable results, enabling me to achieve precise and meaningful outcomes in my research.

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