Active Recombinant Human JAK1(866-end) Protein, N-GST-tagged

• Cat.No.: JAK1-115H
• Product Overview: N-terminal GSTtagged, recombinant, human JAK1 amino acids 866-end, expressed by baculovirus in Sf21 insect cells. Purified using glutathione sepharose. Activated in vitro by autophosphorylation.

Specification

• Description: Janus kinase 1 (JAK1), is a member of a new class of protein-tyrosine kinases (PTK) characterized by the presence of a second phosphotransferase-related domain immediately N-terminal to the PTK domain. The second phosphotransferase domain bears all the hallmarks of a protein kinase, although its structure differs significantly from that of the PTK and threonine/serine kinase family members. JAK1 is a large, widely expressed membrane-associated phosphoprotein. JAK1 is involved in the interferon-α/beta and -γ signal transduction pathways. The reciprocal interdependence between JAK1 and TYK2 activities in the interferon-α pathway, and between JAK1 and JAK2 in the interferon-γ pathway, may reflect a requirement for these kinases in the correct assembly of interferon receptor complexes.
• Source: Sf21
• Species: Human
• Tag: GST
• Protein length: 866-end
• Form: 0.623mg/ml of enzyme in 50mM Tris/HCl pH7.5, 150mM NaCl, 5mM β-glycerophosphate, 0.5mM sodium orthovanadate, 50% v/v glycerol, 0.02% Triton® X-100, 0.5mM EDTA, 2mM DTT. Frozen solution.
• Bio-activity: 76U/mg, where one unit of JAK1, active activity is defined as 1nmol phosphate incorporated into 500 μM (GEEPLYWSFPAKKK) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 61 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSQTSL YKKAGTMDPT 241 HFEKRFLKRI RDLGEGHFGK VELCRYDPEG DNTGEQVAVK SLKPESGGNH IADLKKEIEI 301 LRNLYHENIV KYKGICTEDG GNGIKLIMEF LPSGSLKEYL PKNKNKINLK QQLKYAVQIC 361 KGMDYLGSRQ YVHRDLAARN VLVESEHQVK IGDFGLTKAI ETDKEYYTVK DDRDSPVFWY 421 APECLMQSKF YIASDVWSFG VTLHELLTYC DSDSSPMALF LKMIGPTHGQ MTVTRLVNTL 481 KEGKRLPCPP NCPDEVYQLM RKCWEFQPSN RTSFQNLIEG FEALLK
• Purity: Purity 76% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: JAK1 Janus kinase 1 [ Homo sapiens (human) ]
• Official Symbol: JAK1
• Synonyms: JAK1; Janus kinase 1; JTK3; AIIDE; JAK1A; JAK1B
• Gene ID: 3716
• mRNA Refseq: NM_002227.4
• Protein Refseq: NP_002218.2
• MIM: 147795
• UniProt ID: P23458