Recombinant Human JAK1 293 Cell Lysate

Cat.No. : JAK1-5107HCL
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Description : Antigen standard for Janus kinase 1 (JAK1) is a lysate prepared from HEK293T cells transiently transfected with a TrueORF gene-carrying pCMV plasmid and then lysed in RIPA Buffer. Protein concentration was determined using a colorimetric assay. The antigen control carries a C-terminal Myc/DDK tag for detection.
Source : HEK 293 cells
Species : Human
Components : This product includes 3 vials: 1 vial of gene-specific cell lysate, 1 vial of control vector cell lysate, and 1 vial of loading buffer. Each lysate vial contains 0.1 mg lysate in 0.1 ml (1 mg/ml) of RIPA Buffer (50 mM Tris-HCl pH7.5, 250 mM NaCl, 5 mM EDTA, 50 mM NaF, 1% NP40). The loading buffer vial contains 0.5 ml 2X SDS Loading Buffer (125 mM Tris-Cl, pH6.8, 10% glycerol, 4% SDS, 0.002% Bromophenol blue, 5% beta-mercaptoethanol).
Size : 0.1 mg
Storage Instruction : Store at -80°C. Minimize freeze-thaw cycles. After addition of 2X SDS Loading Buffer, the lysates can be stored at -20°C. Product is guaranteed 6 months from the date of shipment.
Applications : ELISA, WB, IP. WB: Mix equal volume of lysates with 2X SDS Loading Buffer. Boil the mixture for 10 min before loading (for membrane protein lysates, incubate the mixture at room temperature for 30 min). Load 5 ug lysate per lane.
Gene Name : JAK1 Janus kinase 1 [ Homo sapiens ]
Official Symbol : JAK1
Synonyms : JAK1; Janus kinase 1; JAK1B; tyrosine-protein kinase JAK1; JAK1A; JTK3;
Gene ID : 3716
mRNA Refseq : NM_002227
Protein Refseq : NP_002218
MIM : 147795
UniProt ID : P23458
Chromosome Location : 1p32.3-p31.3
Pathway : Antiviral mechanism by IFN-stimulated genes, organism-specific biosystem; Cytokine Signaling in Immune system, organism-specific biosystem; EGFR1 Signaling Pathway, organism-specific biosystem; HTLV-I infection, organism-specific biosystem; HTLV-I infection, conserved biosystem; Hepatitis C, organism-specific biosystem; Hepatitis C, conserved biosystem;
Function : ATP binding; growth hormone receptor binding; non-membrane spanning protein tyrosine kinase activity; nucleotide binding; protein binding; protein tyrosine kinase activity; protein tyrosine kinase activity;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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03/13/2022

    Top-notch purity, impressive results.

    09/30/2021

      Excellent stability, very useful.

      11/17/2020

        Fast and reliable delivery.

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