Active Recombinant Human PLK3(19-301) Protein, N-His-tagged

• Cat.No.: PLK3-202H
• Product Overview: N-terminal 6Histagged recombinant human Plk3 residues 19–301, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: Cytokine-inducible kinase is a putative serine/threonine kinase. CNK contains both a catalytic domain and a putative regulatory domain. It may play a role in regulation of cell cycle progression and tumorigenesis.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 19-301 aa
• Form: 0.420mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 20mM β-glycerophosphate, 0.25mM sodium orthovanadate, 10mM NaF, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2- mercaptoethanol. Frozen solution.
• Bio-activity: 1532U/mg, where one unit of Plk3, active activity is defined as 1nmol phosphate incorporated into 2mg/ml casein per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 36 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMSLGS SGRTYLKGRL LGKGGFARCY EATDTETGSA 61 YAVKVIPQSR VAKPHQREKI LNEIELHRDL QHRHIVRFSH HFEDADNIYI FLELCSRKSL 121 AHIWKARHTL LEPEVRYYLR QILSGLKYLH QRGILHRDLK LGNFFITENM ELKVGDFGLA 181 ARLEPPEQRK KTICGTPNYV APEVLLRQGH GPEADVWSLG CVMYTLLCGS PPFETADLKE 241 TYRCIKQVHY TLPASLSLPA RQLLAAILRA SPRDRPSIDQ ILRHDFFTKG YTPDRLPISS 301 CVTVPDLTPP NPA
• Purity: Purity 77% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PLK3 polo like kinase 3 [ Homo sapiens (human) ]
• Official Symbol: PLK3
• Synonyms: PLK3; polo like kinase 3; CNK; FNK; PRK; PLK-3
• Gene ID: 1263
• mRNA Refseq: NM_004073.4
• Protein Refseq: NP_004064.2
• MIM: 602913
• UniProt ID: Q9H4B4