Recombinant Human Polo-like Kinase 3 (Drosophila), His-tagged

• Cat.No.: PLK3-566H
• Product Overview: Recombinant Human Plk3 (a.a. 58-339) with N-terminal His tag expressed in aBaculovirus infected Sf9 cellexpression system. MW=36.7 kDa.

Specification

• Species: Human
• Source: Sf9 Cells
• Tag: His
• Protein Length: 58-339 a.a.
• Description: Polo-like kinase 3 (Drosophila), also known as PLK3, is an enzyme which in humans is encoded by the PLK3 gene. Cytokine-inducible kinase is a putative serine/threonine kinase. CNK contains both a catalytic domain and a putative regulatory domain. It may play a role in regulation of cell cycle progression and tumorigenesis. PLK3 has been shown to interact with CDC25C, CHEK2 and P53.
• Application: Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
• Specific Activity: 500 U/mg. One unit is defined as the amount of enzyme that will phosphorylate 1 nmol of casein substrate per minute at pH 7.4 and 30°C. Assay buffer: 50 mM HEPES, pH 7.4, 3 mM MgCl2, 3 mM MnCl2, 1 mM DTT, 3 uM Na-orthovanadate, 0.5 mM ATP.
• Purity: >80% by SDS-PAGE.
• Formulated In: 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 50% glycerol, and 3 mM DTT.
• Stability: >6 months at -80°C.

Gene Information

• Gene Name: PLK3 polo-like kinase 3 (Drosophila) [ Homo sapiens ]
• Synonyms: polo-like kinase 3 (Drosophila); CNK; FNK; PRK; PLK3; serine/threonine-protein kinase PLK3; PLK-3; FGF-inducible kinase; cytokine-inducible kinase; proliferation-related kinase; cytokine-inducible serine/threonine-protein kinase; Polo-like kinase 3; Proliferation-related kinase; Cytokine-inducible serine/threonine-protein kinase; EC 2.7.11.21
• Gene ID: 1263
• mRNA Refseq: NM_004073.2
• Protein Refseq: NP_004064.2
• MIM: 602913
• UniProt ID: Q9H4B4
• Chromosome Location: 1p34.1
• Function: ATP binding; nucleotide binding; polo kinase kinase activity; protein binding; protein serine/threonine kinase activity; transferase activity