Active Recombinant Human PTK2(411-686) Protein, N-His-tagged

• Cat.No.: PTK2-78H
• Product Overview: N-terminal 6His-tagged, recombinant, human FAK, amino acids 411–686, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: PTK2 (Protein Tyrosine Kinase 2, formerly FAK or Focal Adhesion Associated Protein Tyrosine Kinase) is a widely expressed non-receptor involved in the structure and function of focal adhesions, which are critical for cellular integrity by maintaining cell-cell and cell-matrix interactions. Integrin-mediated cell adhesion causes activation of MAP kinases and increased tyrosine phosphorylation of PTK2. Autophosphorylation of PTK2 leads to the binding of SH2-domain proteins including Src-family kinases and the Grb2-Sos complex, then activate Ras cascade and ultimately to mitogen activated protein (MAP) kinase activation.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 411-686 aa
• Form: 2.178mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 79U/mg, where one unit of FAK activity is defined as 1nmol phosphate incorporated into 100μM (EEEEYEEEEEEYYIIEEEEEEYEEEEEEYYEEE EEEKKK) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 35.5 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF STRDYEIQRE RIELGRCIGE GQFGDVHQGI 61 YMSPENPALA VAIKTCKNCT SDSVREKFLQ EALTMRQFDH PHIVKLIGVI TENPVWIIME 121 LCTLGELRSF LQVRKYSLDL ASLILYAYQL STALAYLESK RFVHRDIAAR NVLVSSNDCV 181 KLGDFGLSRY MEDSTYYKAS KGKLPIKWMA PESINFRRFT SASDVWMFGV CMWEILMHGV 241 KPFQGVKNND VIGRIENGER LPMPPNCPPT LYSLMTKCWA YDPSRRPRFT ELKAQLSTIL 301 EEEKAQ
• Purity: Purity 96% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: PTK2 protein tyrosine kinase 2 [ Homo sapiens (human) ]
• Official Symbol: PTK2
• Synonyms: PTK2; protein tyrosine kinase 2; FAK; FADK; FAK1; FRNK; FADK 1; PPP1R71; p125FAK; pp125FAK
• Gene ID: 5747
• mRNA Refseq: NM_001199649.2
• Protein Refseq: NP_001186578.1
• MIM: 600758
• UniProt ID: Q05397