| Description : |
MST3 (Mammalian sterile 20-like kinase 3) is a serine/threonine protein kinase that belongs to the sterile 20 (STE20) family of kinases. It is involved in a variety of cellular processes, including cell cycle progression, cell migration, and apoptosis. Research has suggested that MST3 plays a role in several diseases, including cancer, neurodegenerative diseases, and cardiovascular diseases. It has been shown to promote cancer cell proliferation and survival, and inhibition of MST3 has been suggested as a potential cancer therapy. Additionally, MST3 has been implicated in the regulation of neuronal survival and synaptic plasticity, making it a potential target for the treatment of neurodegenerative diseases such as Alzheimer's and Parkinson's disease. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
GST |
| Protein length : |
1-304 aa |
| Form : |
2.92mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
84U/mg, where one unit of MST3 activity is defined as 1nmol phosphate incorporated into 250μM PKCtide (ERMRPRKRQGSVRRRV) per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
61.2 kDa |
| AA Sequence : |
1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPEF MAHSPVQSGL 241 PGMQNLKADP EELFTKLEKI GKGSFGEVFK GIDNRTQKVV AIKIIDLEEA EDEIEDIQQE 301 ITVLSQCDSP YVTKYYGSYL KDTKLWIIME YLGGGSALDL LEPGPLDETQ IATILREILK 361 GLDYLHSEKK IHRDIKAANV LLSEHGEVKL ADFGVAGQLT DTQIKRNTFV GTPFWMAPEV 421 IKQSAYDSKA DIWSLGITAI ELARGEPPHS ELHPMKVLFL IPKNNPPTLE GNYSKPLKEF 481 VEACLNKEPS FRPTAKELLK HKFILRNAKK TSYLTELIDR YKRWKAEQSH DDSS |
| Purity : |
Purity 98.5% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
