Active Recombinant Human STK26(4-304) Protein, N-His-tagged

• Cat.No.: STK26-166H
• Product Overview: N-terminal 6Histagged, recombinant, human MST4 amino acids 4-304 expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: MST4 is a member of the GCK group III family of kinases, which are a subset of the Ste20-like kinases. This protein kinase contains an amino-terminal kinase domain, and a carboxy-terminal regulatory domain that mediates homodimeriziaton. The protein kinase localizes to the Golgi apparatus and is specifically activated by binding to the Golgi matrix protein GM130. It is also cleaved by caspase-3 in vitro, and may function in the apoptotic pathway.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 4-304 aa
• Form: 1.510mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 584U/mg, where one unit of MST4, active activity is defined as 1nmol phosphate incorporated into 250μM (RLGRDKYKTLRQIRQ) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 38 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF SPVAVQVPGM QNNIADPEEL FTKLERIGKG 61 SFGEVFKGID NRTQQVVAIK IIDLEEAEDE IEDIQQEITV LSQCDSSYVT KYYGSYLKGS 121 KLWIIMEYLG GGSALDLLRA GPFDEFQIAT MLKEILKGLD YLHSEKKIHR DIKAANVLLS 181 EQGDVKLADF GVAGQLTDTQ IKRNTFVGTP FWMAPEVIQQ SAYDSKADIW SLGITAIELA 241 KGEPPNSDMH PMRVLFLIPK NNPPTLVGDF TKSFKEFIDA CLNKDPSFRP TAKELLKHKF 301 IVKNSKKTSY LTELIDRFKR WKAEGHSDDE S
• Purity: Purity 87% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: STK26 serine/threonine kinase 26 [ Homo sapiens (human) ]
• Official Symbol: STK26
• Synonyms: STK26; serine/threonine kinase 26; MASK; MST4
• Gene ID: 51765
• mRNA Refseq: NM_016542.4
• Protein Refseq: NP_057626.2
• MIM: 300547
• UniProt ID: Q9P289