"ALKBH2" Related Products

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Recombiant Hmuan AlkB, Alkylation Repair Homolog 2, His-tagged

Cat. No.: ALKBH2-977H
Product Overview: Recombinant human ALKBH2 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.
Description: Alpha-ketoglutarate-dependent dioxygenasealkB homolog 2, also known ALKBH2, belongs to the ALKB family. Expressed in heart, colon, liver, testis, ovary, prostate and small intestine, ALKBH2 uses iron as a cofactor and functions as a dioxygenase that catalyzes the repair of alkylated DNA and RNA containing 1-methyladenine and 3-meth-ylcytosine. ALKBH2 is functionally activated by ascorbate and requires oxygen and α-ketoglutarate for enzymatic activity.
Formulation: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol
Molecular Weight: 31.4kDa (281aa), confirmed by MALDI-TOF
Purity: > 90% by SDS – PAGE
Sequences of amino acids: MGSSHHHHH HSSGLVPRGSHMDRFLVKGAQ GGLLRKQEEQ EPTGEEPAVL GGDKESTRKR PRREAPGNGG HSAGPSWRHI RAEGLDCSYT VLFGKAEADE IFQELEKEVE YFTGALARVQ VFGKWHSVPR KQATYGDAGL TYTFSGLTLS PKPWIPVLER IRDHVSGVTG QTFNFVLINR YKDGCDHIGE HRDDERELAP GSPIASVSFG ACRDFVFRHK DSRGKSPSRR VAVVRLPLAH GSLLMMNHPT NTHWYHSLPV RKKVLAPRVN LTFRKILLTK K
concentration: 0.5 mg/ml (determined by Bradford assay)
Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C.Avoid repeated freezing and thawing cycles. Avoid repeated freezing and thawing cycles.
Gene Name: ALKBH2 alkB, alkylation repair homolog 2 (E. coli) [ Homo sapiens ]
Synonyms: ALKBH2;alkB, alkylation repair homolog 2 (E. coli);ABH2; FLJ99103; MGC90512; alpha-ketoglutarate-dependent dioxygenasealkB homolog 2;oxy DC1;2OG-Fe(II) oxy DC1;alkylated DNA repair protein alkB homolog 2;EC 1.14.11.
Gene ID: 121642
MIM: 610602
mRNA Refseq: NM_001001655
Protein Refseq: NP_001001655
UniProt ID: Q6NS38
Chromosome Location: 12q24.11
Function: 2-oxoglutarate-dependent dioxygenase activity;DNA-N1-methyladenine dioxygenase activity;cytosine C-5 DNA demethylase activity;damaged DNA binding;ferrous iron binding;metal ion binding;oxidoreductase activity;oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen.

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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