Recombinant Mouse Alkbh2 Protein, Myc/DDK-tagged

• Cat.No.: Alkbh2-1600M
• Product Overview: Purified recombinant protein of mouse full-length alkB homolog 2, alpha-ketoglutarate-dependent dioxygenase (Alkbh2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Dioxygenase that repairs alkylated DNA and RNA containing 1-methyladenine and 3-methylcytosine by oxidative demethylation. Can also repair alkylated DNA containing 1-ethenoadenine. Has strong preference for double-stranded DNA. Has low efficiency with single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron.
• Molecular Mass: 27.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Alkbh2 alkB homolog 2, alpha-ketoglutarate-dependent dioxygenase [ Mus musculus (house mouse) ]
• Official Symbol: Alkbh2
• Synonyms: ALKBH2; alkB, alkylation repair homolog 2 (E. coli); alpha-ketoglutarate-dependent dioxygenase alkB homolog 2; alkylated DNA repair protein alkB homolog 2; Abh2; mABH2; AU016977; 9530023G02
• Gene ID: 231642
• mRNA Refseq: NM_175016
• Protein Refseq: NP_778181
• UniProt ID: Q6P6J4