Active Recombinant Human GSF2

• Cat.No.: GSF2-110H
• Product Overview: Granulocyte Macrophage Colony Stimulating Factor Human Recombinant produced in E.coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14477 Dalton. GM-CSF is purified by proprietary chromatographic techniques.

Specification

• Description: The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of granulocytes and macrophages. The active form of the protein is found extracellularly as a homodimer. This gene has been localized to a cluster of related genes at chromosome region 5q31, which is known to be associated with interstitial deletions in the 5q- syndrome and acute myelogenous leukemia. Other genes in the cluster include those encoding interleukins 4, 5, and 13.
• Source: E. coli
• Species: Human
• Form: GM-CSF was lyophilized after extensive dialysis against 2 mM sodium phosphate buffer pH= 7.4±0.1. Sterile filtered White lyophilized powder.
• Bio-activity: The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is< 0.1 ng/ml, corresponding to a specific activity of 11.1×106>
• AA Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Pro-Ala-Arg-Ser. N-terminal methionine has been completely removed enzymatically.
• Purity: Greater than 98.0% as determined by RP-HPLC and SDS-PAGE
• Storage: Lyophilized Granulocyte Macrophage Colony Stimulating Factor although stable at room temperature for 3 weeks, should be stored desiccated below –18°C. Upon reconstitution GMCSF should be stored at 4°C between 2–7 days and for future use below –18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
• Shipping: At ambient temperature. Upon receipt, store the product at the temperature recommended below.

Gene Information

• Gene Name: CSF2 colony stimulating factor 2 (granulocyte-macrophage) [ Homo sapiens (human) ]
• Official Symbol: CSF2
• Synonyms: CSF2; GMCSF; colony stimulating factor 2 (granulocyte-macrophage); granulocyte-macrophage colony-stimulating factor; CSF; molgramostin; sargramostim
• Gene ID: 1437
• mRNA Refseq: NM_000758
• Protein Refseq: NP_000749
• MIM: 138960
• UniProt ID: P04141
• Chromosome Location: 5q31.1
• Pathway: Calcineurin-regulated NFAT-dependent transcription in lymphocytes; Cytokine Signaling in Immune system; Fc epsilon RI signaling pathway
• Function: cytokine activity; granulocyte macrophage colony-stimulating factor receptor binding; growth factor activity