| Cat. No.: | TXNRD1-2497H |
| Product Overview: | Recombinant human TXNRD1 protein, fused to His-tag at N-terminus, was expressed inE.coliand purified by using conventional chromatography techniques. MW = 55.7 kDa (508aa). |
| Description: | TXNRD1, also known as Thioredoxin reductase 1, is a part of a selenium-containing pyridine nucleotide-disulphide oxidoreductase family, which has a conserved catalytic site of Cys-Val-Asn-Val-Gly-Cys. This protein reduces thioredoxins as well as other substrates, and plays a role in selenium metabolism and protection against oxidative stress. Inhibition of TXNRD1 activity may provide for potential treatments of cancer, AIDS and other autoimmune diseases as well as bacterial infections and parasitic diseases. |
| Source: | E.coli. |
| Form: | Liquid. In Phosphate Buffered Saline pH7.4 containing 10% glycerol. |
| Purity: | > 95% by SDS – PAGE. |
| Concentration: | 0.5 mg/ml (determined by Bradford assay). |
| Sequences Of Amino Acids: | MGSSHHHHHH SSGLVPRGSH MYDYDLIIIG GGSGGLAAAK EAAQYGKKVM VLDFVTPTPL GTRWGLGGTC VNVGCIPKKL MHQAALLGQA LQDSRNYGWK VEETVKHDWD RMIEAVQNHI GSLNWGYRVA LREKKVVYEN AYGQFIGPHR IKATNNKGKE KIYSAERFLI ATGERPRYLG IPGDKEYCIS SDDLFSLPYC PGKTLVVGAS YVALECAGFL AGIGLDVTVM VRSILLRGFD QDMANKIGEH MEEHGIKFIR QFVPIKVEQI EAGTPGRLRV VAQSTNSEEI IEGEYNTVML AIGRDACTRK IGLETVGVKI NEKTGKIPVT DEEQTNVPYI YAIGDILEDK VELTPVAIQA GRLLAQRLYA GSTVKCDYEN VPTTVFTPLE YGACGLSEEK AVEKFGEENI EVYHSYFWPL EWTIPSRDNN KCYAKIICNT KDNERVVGFH VLGPNAGEVT QGFAAALKCG LTKKQLDSTI GIHPVCAEVF TTLSVTKRSG ASILQAGC. |
| Biological activity: | Specific activity is 0.48-0.6 units/ml, and was measured in a coupled assay with DTNB and NADPH. The amount of TNB generated by NADPH was measured in absorbance at 412 nm. |
| Activity Assay: | 1. Prepare a 0.7ml reaction mixture into a suitable container: The finalconcentrations are 100mM potassium phosphate, 10mM EDTA, 0.2mM beta-NADPH, 0.05% BSA, 0.014% (w/v) thioredoxin, 5mM DTNB, 5ug, human TRXNRD1. 2. Equilibrate to 25C and monitor the A412nm until the value is constant using a spectrophotometer. 3. Add 35ul of 100mM DTNB into reaction mixture and mix immediately. 4. Record the increase in A412nm for 2 minutes. |
| Storage: | can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles. |
| Gene Name: | TXNRD1 thioredoxin reductase 1 [ Homo sapiens ] |
| Synonyms: | thioredoxin reductase 1; TR; TR1; TXNR; TRXR1; GRIM-12; MGC9145; TXNRD1; thioredoxin reductase 1, cytoplasmic; oxidoreductase; thioredoxin reductase TR1; thioredoxin reductase GRIM-12; KM-102-derived reductase-like factor; gene associated with retinoic and IFN-induced mortality 12 protein; gene associated with retinoic and interferon-induced mortality 12 protein; Trxr1; EC 1.8.1.9; Gene associated with retinoid-IFN-induced mortality 12 protein; Thioredoxin reductase TR1 |
| Gene ID: | 7296 |
| mRNA Refseq: | NM_001093771 |
| Protein Refseq: | NP_001087240 |
| MIM: | 601112 |
| UniProt ID: | Q16881 |
| Chromosome Location: | 12q23-q24.1 |
| Pathway: | Pyrimidine metabolism; Metabolism of nucleotides |
| Function: | FAD binding; NADP or NADPH binding; electron carrier activity; oxidoreductase activity; protein disulfide oxidoreductase activity; thioredoxin-disulfide reductase activity |
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