Recombinant Rat Txnrd1 Protein

Cat.No. : Txnrd1-001R
Product Overview : Active recombinant rat TrxR1 protein (3-499) without tag was expressed in E. coli.
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Description : The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes an ubiquitously expressed, cytosolic form of TrxR, which functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing, primarily at the 5' end, results in transcript variants encoding same or different isoforms.
Source : E. coli
Species : Rat
Molecular Mass : 54.5 kDa
Protein length : 3-499
Purity : ≥90% as determined by SDS-PAGE
Unit Definition : One unit is defined as the NADPH-dependent production of 2 mmol of 2-nitro-5-thiobenzoate per minute at 22 centigrade in 50 mM potassium phosphate, pH 7.0, with 50 mM potassium chloride, 1 mM EDTA, and 0.2 mg/mL BSA. For inhibition, the selective TrxR1 inhibitor sodium aurothiomalate (ATM) was used at a concentration of 20 mM.
Applications : WB, Enzyme activity assay
Stability : ≥ 1 year
Storage : At -80 centigrade.
Storage Buffer : 50 mM Tris-HCl, pH 7.4, with 2 mM EDTA and 10% glycerol
FACS: Lane 1: MW Markers<br />Lane 2: Thioredoxin Reductase 1 (2 μg)<br />Lane 3: Thioredoxin Reductase 1 (4 μg)<br />rTrxR1 concentration was determined spectrophotometrically by FAD absorption at 463 nm(ε=13600M-1cm-1)
Gene Name : Txnrd1 thioredoxin reductase 1 [ Rattus norvegicus (Norway rat) ]
Official Symbol : Txnrd1
Synonyms : Txnrd1; thioredoxin reductase 1; Tr; thioredoxin reductase 1, cytoplasmic; NADPH-dependent thioredoxin reductase; selenoprotein oxidoreductase; thioredoxin reductase TR1; EC
Gene ID : 58819
mRNA Refseq : NM_031614
Protein Refseq : NP_113802
UniProt ID : O89049

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