| Species : |
Rat |
| Source : |
E.coli |
| Protein Length : |
3-499 |
| Description : |
The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes an ubiquitously expressed, cytosolic form of TrxR, which functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing, primarily at the 5' end, results in transcript variants encoding same or different isoforms. |
| Molecular Mass : |
54.5 kDa |
| Purity : |
≥90% as determined by SDS-PAGE |
| Unit Definition : |
One unit is defined as the NADPH-dependent production of 2 mmol of 2-nitro-5-thiobenzoate per minute at 22 centigrade in 50 mM potassium phosphate, pH 7.0, with 50 mM potassium chloride, 1 mM EDTA, and 0.2 mg/mL BSA. For inhibition, the selective TrxR1 inhibitor sodium aurothiomalate (ATM) was used at a concentration of 20 mM. |
| Applications : |
WB, Enzyme activity assay |
| Stability : |
≥ 1 year |
| Storage : |
At -80 centigrade. |
| Storage Buffer : |
50 mM Tris-HCl, pH 7.4, with 2 mM EDTA and 10% glycerol |
