Recombinant Human Thioredoxin reductase 1 Protein, His-tagged

• Cat.No.: TXNRD1-32H
• Product Overview: Recombinant human TXNRD1 protein with a His-tag was expressed in HEK293

Specification

• Description: The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes an ubiquitously expressed, cytosolic form of TrxR, which functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing, primarily at the 5' end, results in transcript variants encoding same or different isoforms, including a glutaredoxin-containing isoform that is predominantly expressed in testis.
• Source: HEK293
• Species: Human
• Tag: His
• Molecular Mass: The protein has a calculated MW of 72 kDa.
• AA Sequence: HHHHHHHHMGCAEGKAVAAAAPTEL QTKGKNGDGRRRSAKDHHPGKTLPE NPAGFTSTATADSRALLQAYIDGHS VVIFSRSTCTRCTEVKKLFKSLCVP YFVLELDQTEDGRALEGTLSELAAE TDLPVVFVKQRKIGGHGPTLKAYQE GRLQKLLKMNGPEDLPKSYDYDLII IGGGSGGLAAAKEAAQYGKKVMVLD FVTPTPLGTRWGLGGTCVNVGCIPK KLMHQAALLGQALQDSRNYGWKVEE TVKHDWDRMIEAVQNHIGSLNWGYR VALREKKVVYENAYGQFIGPHRIKA TNNKGKEKIYSAERFLIATGERPRY LGIPGDKEYCISSDDLFSLPYCPGK TLVVGASYVALECAGFLAGIGLDVT VMVRSILLRGFDQDMANKIGEHMEE HGIKFIRQFVPIKVEQIEAGTPGRL RVVAQSTNSEEIIEGEYNTVMLAIG RDACTRKIGLETVGVKINEKTGKIP VTDEEQTNVPYIYAIGDILEDKVEL TPVAIQAGRLLAQRLYAGSTVKCDY ENVPTTVFTPLEYGACGLSEEKAVE KFGEENIEVYHSYFWPLEWTIPSRD NNKCYAKIICNTKDNERVVGFHVLG PNAGEVTQGFAAALKCGLTKKQLDS TIGIHPVCAEVFTTLSVTKRSGASI LQAGCCG
• Purity: >90% by SDS-PAGE
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Concentration: 3.71 mg/mL
• Storage Buffer: 20 mM Tris +150 mM NaCl, pH8.4

Gene Information

• Gene Name: TXNRD1 thioredoxin reductase 1 [ Homo sapiens (human) ]
• Official Symbol: TXNRD1
• Synonyms: TXNRD1; thioredoxin reductase 1; thioredoxin reductase 1, cytoplasmic; GRIM 12; Trxr1; TXNR; oxidoreductase; thioredoxin reductase TR1; thioredoxin reductase GRIM-12; KM-102-derived reductase-like factor; gene associated with retinoic and IFN-induced mortality 12 protein; gene associated with retinoic and interferon-induced mortality 12 protein; TR; TR1; TRXR1; GRIM-12; MGC9145
• Gene ID: 7296
• mRNA Refseq: NM_001093771
• Protein Refseq: NP_001087240
• MIM: 601112
• UniProt ID: Q16881