Recombinant Human TXNRD1 Protein

• Cat.No.: TXNRD1-22H
• Product Overview: Recombinant Human Thioredoxin Reductase 1 (Non-tagged, 499 residues)

Specification

• Species: Human
• Tag: Non
• Description: The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes an ubiquitously expressed, cytosolic form of TrxR, which functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing, primarily at the 5' end, results in transcript variants encoding same or different isoforms, including a glutaredoxin-containing isoform that is predominantly expressed in testis.
• Purity: ≥ 95% as judged by gel analysis
• Unit Definition: 1 U of enzyme reduces 1 μmol DTNB per min in 0.5 mL standard DTNB assay containing 2.5 mM DTNB and 0.3 mM NADPH in TE buffer (50 mM Tris-HCl, 2 mM EDTA, pH 7.5)
• Applications: DTNB assay; Insulin-coupled Thioredoxin assay; Inhibitor screening; etc.
• Notes: Centrifuge the tube briefly before opening the lid
• Storage: Store at -20 centigrade upon delivery.
• Concentration: 1 mg/mL
• Storage Buffer: TE buffer with 50% glycerol.
• Shipping: Shipped in cold pack by courier service.

Gene Information

• Gene Name: TXNRD1 thioredoxin reductase 1 [ Homo sapiens (human) ]
• Official Symbol: TXNRD1
• Synonyms: TXNRD1; thioredoxin reductase 1; TR; TR1; TXNR; TRXR1; GRIM-12; thioredoxin reductase 1, cytoplasmic; KM-102-derived reductase-like factor; gene associated with retinoic and IFN-induced mortality 12 protein; gene associated with retinoic and interferon-induced mortality 12 protein; oxidoreductase; peroxidase TXNRD1; selenoprotein TXNRD1; testis tissue sperm-binding protein Li 46a; thioredoxin reductase GRIM-12; thioredoxin reductase TR1; EC 1.11.1.2; EC 1.8.1.9
• Gene ID: 7296
• mRNA Refseq: NM_182743
• Protein Refseq: NP_877420
• MIM: 601112
• UniProt ID: Q16881