Recombinant Rat Txnrd1 Protein

• Cat.No.: Txnrd1-021R
• Product Overview: Recombinant Rat Txnrd1 protein(1021-1323) without tag was expressed in E.coli

Specification

• Description: The protein encoded by this gene belongs to the pyridine nucleotide-disulfide oxidoreductase family, and is a member of the thioredoxin (Trx) system. Three thioredoxin reductase (TrxR) isozymes are found in mammals. TrxRs are selenocysteine-containing flavoenzymes, which reduce thioredoxins, as well as other substrates, and play a key role in redox homoeostasis. This gene encodes an ubiquitously expressed, cytosolic form of TrxR, which functions as a homodimer containing FAD, and selenocysteine (Sec) at the active site. Sec is encoded by UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Alternative splicing, primarily at the 5' end, results in transcript variants encoding same or different isoforms.
• Source: E. coli
• Species: Rat
• Unit Definition: One ampoule contains 100 µl of recombinant rat thioredoxin reductase 1 (TrxR1) in 50 mM Tris-HCl, 1 mM EDTA, pH 7.5 and 50 percent glycerol with a total activity of 5 units and 0.15 mg of active enzyme protein. One unit of enzyme catalyses oxidation of one micromol of NADPH per min under standard mammalian TrxR assay conditions in the presence of 5 mM DTNB.
• Stability: ≥ 6 months
• Storage: -20 centigrade
• Shipping: Room Temperature in continental US, may vary elsewhere.
• Protein length: 1021-1323

Gene Information

• Gene Name: Txnrd1 thioredoxin reductase 1 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Txnrd1
• Synonyms: Txnrd1; thioredoxin reductase 1; Tr; thioredoxin reductase 1, cytoplasmic; NADPH-dependent thioredoxin reductase; selenoprotein oxidoreductase; thioredoxin reductase TR1; EC 1.8.1.9
• Gene ID: 58819
• mRNA Refseq: NM_001351981
• Protein Refseq: NP_001338910