Recombinant Human EPO protein

• Cat.No.: EPO-517H
• Product Overview: Recombinant Human EPO protein (Ala28-Arg193) carries no tag and was expressed in human 293 cells (HEK293).

Specification

• Description: Human Erythropoietin (EPO) is also known as EP, erythropoetin or erthropoyetin, and is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. EPO is a cytokine for erythrocyte (red blood cell) precursors in the bone marrow. is synthesized by renal peritubular cells in adults, with a small amount being produced in the liver. Regulation is believed to rely on a feed-back mechanism measuring blood oxygenation. Constitutively synthesized transcription factors for EPO, known as hypoxia-inducible factors (HIFs), are hydroxylated and proteosomally digested in the presence of oxygen. It binds to the erythropoietin receptor (EpoR) on the red cell surface and activates a JAK2 cascade. Erythropoietin has its primary effect on red blood cells by promoting red blood cell survival through protecting these cells from apoptosis. It also cooperates with various growth factors involved in the development of precursor red cells. EPO has a range of actions including vasoconstriction-dependent hypertension, stimulating angiogenesis, and inducing proliferation of smooth muscle fibers. It has also been shown that erythropoietin can increase iron absorption by suppressing the hormone hepcidin. Erythropoietin has been shown to interact with the Erythropoietin receptor as its mechanism of action within the body. erythropoietin plays an important role in the brain's response to neuronal injury. EPO is also involved in the wound healing process.
• Source: HEK293
• Species: Human
• Form: Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
• Molecular Mass: The protein has a calculated MW of 18.4 kDa. The protein migrates as 30-37 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Protein length: 166
• Endotoxin: Less than 1.0 EU per ug by the LAL method.
• Purity: >97% as determined by SDS-PAGE.
• Storage: For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower.
  Please avoid repeated freeze-thaw cycles.
  This product is stable after storage at:
  -20 centigrade to -70 centigrade for 12 months in lyophilized state;
  -70 centigrade for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: EPO
• Official Symbol: EPO
• Synonyms: EPO; erythropoietin; EP; epoetin; MVCD2; MGC138142
• Gene ID: 2056
• mRNA Refseq: NM_000799
• Protein Refseq: NP_000790
• MIM: 133170
• UniProt ID: P01588

Reviews

12/28/2021

Stability is truly remarkable, can maintain its activity even under extreme conditions.

08/31/2021

Can catalyze reactions even under extreme conditions.

02/03/2019

This protein's repeatability is truly exceptional, consistently delivering reliable results even under challenging conditions.

Q&As

What is the relationship between EPO protein and cardiovascular disease?

EPO protein can promote the renewal and repair of heart blood vessels, and has the effect of protecting the heart from ischemia-reperfusion damage.

How does EPO protein regulate erythropoiesis?

By binding to receptors, EPO proteins activate signaling pathways that promote the proliferation and differentiation of hematopoietic stem cells in bone marrow into erythrocytes.

How about the clinical use of EPO protein?

EPO protein can be used by injection or oral, and the specific method and dosage need to be determined according to the condition and the doctor's advice.

What are the implications of EPO protein research for the future of treating anemia?

The study of EPO protein helps to understand the regulatory mechanism of erythropoiesis and provides new ideas and methods for the treatment of anemia, such as developing more effective EPO protein analogues or improving existing treatment regimens.

How is EPO protein treated for anemia?

This protein stimulates the bone marrow to produce more red blood cells, thereby increasing hemoglobin levels and treating anemia.

What is the role of EPO protein in kidney disease?

In kidney disease, due to impaired kidney function, the production of EPO protein decreases, leading to anemia. Supplementation with exogenous EPO protein can help correct anemia caused by kidney problems.