Recombinant Human RBBP4 cell lysate

• Cat.No.: RBBP4-650HCL
• Product Overview: Human RBBP4 / RBAP48 derived in Baculovirus-Insect cells. The whole cell lysate is provided in 1X Sample Buffer.Browse all transfected cell lysate positive controls

Specification

• Source: Baculovirus-Insect Cells
• Species: Human
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.

Gene Information

• Gene Name: RBBP4 retinoblastoma binding protein 4 [ Homo sapiens ]
• Official Symbol: RBBP4
• Synonyms: RBBP4; retinoblastoma binding protein 4; histone-binding protein RBBP4; NURF55; RbAp48; RBBP-4; CAF-I p48; CAF-1 subunit C; CAF-I 48 kDa subunit; MSI1 protein homolog; retinoblastoma-binding protein 4; retinoblastoma-binding protein p48; chromatin assembly factor 1 subunit C; chromatin assembly factor I p48 subunit; chromatin assembly factor/CAF-1 p48 subunit; nucleosome-remodeling factor subunit RBAP48; RBAP48
• Gene ID: 5928
• mRNA Refseq: NM_001135255
• Protein Refseq: NP_001128727
• MIM: 602923
• UniProt ID: Q09028
• Chromosome Location: 1p35.1
• Pathway: Cell Cycle, organism-specific biosystem; Cell Cycle, Mitotic, organism-specific biosystem; Chromosome Maintenance, organism-specific biosystem; Deposition of New CENPA-containing Nucleosomes at the Centromere, organism-specific biosystem; E2F transcription factor network, organism-specific biosystem; G0 and Early G1, organism-specific biosystem; Hedgehog signaling events mediated by Gli proteins, organism-specific biosystem
• Function: contributes_to DNA-dependent ATPase activity; histone binding; histone deacetylase binding; protein binding

Reviews

12/20/2020

They can offer specialized formulations, concentrations, or modifications of RBBP4 protein to suit unique experimental requirements.

04/19/2018

The manufacturer can further support researchers by providing customized solutions tailored to their specific needs.

06/20/2016

Collaborative discussions with the manufacturer can also help optimize experimental protocols and provide insights into the best practices in working with RBBP4.

Q&As

How is RBBP4 involved in cell differentiation?

RBBP4 participates in the regulation of genes involved in cell differentiation, which is crucial for the development and maintenance of tissues.

Are there any clinical applications for RBBP4 in cancer treatment?

Research into targeting RBBP4 in cancer therapy is ongoing, as it may help in developing novel treatments for certain types of cancer.

How can RBBP4 influence cancer progression?

RBBP4's role in gene regulation can impact the expression of genes involved in cell proliferation and differentiation, affecting cancer progression.

Can RBBP4 be used as a diagnostic marker for cancer?

RBBP4 is not typically used as a standalone diagnostic marker, but its expression levels can be studied to understand the molecular mechanisms underlying cancer.

What other diseases or conditions are associated with RBBP4?

RBBP4's role in gene regulation implicates it in various diseases and conditions beyond cancer, including neurological disorders and developmental diseases.