Recombinant Human CSF1, His-tagged

• Cat.No.: CSF1-26835TH
• Product Overview: Recombinant fragment, corresponding to amino acids 87-235 of Human MCSF with an additional N terminal Arginine with His tag produced in the endosperm tissue of barley grain ; 149 amino acids , 21 kDa.

Specification

• Description: The protein encoded by this gene is a cytokine that controls the production, differentiation, and function of macrophages. The active form of the protein is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. The encoded protein may be involved in development of the placenta. Alternate splicing results in multiple transcript variants.
• Conjugation: HIS
• Biological activity: Bioactivity:Bioactivity is assayed by measuring the dose dependent effect of Human MCSF on proliferation of M-NFS-60 cells. The ED50 for this batch is 5.16 ng/ml, according to this bioassay. Activity range is 4.13-6.19 ng/ml.
• Form: Lyophilised:It is recommended to reconstitute the lyophilized protein in sterile phosphate-buffered saline to a concentration of no less than 100 μg/ml. For long term storage of the reconstituted solution it is recommended to add a carrier protein (0.1% H
• Purity: >95% by SDS-PAGE
• Storage buffer: Preservative: NoneConstituents: 5mM Tris HCl, 80 mM Arginine, pH 8.0
• Storage: Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

Gene Information

• Gene Name: CSF1 colony stimulating factor 1 (macrophage) [ Homo sapiens ]
• Official Symbol: CSF1
• Synonyms: CSF1; colony stimulating factor 1 (macrophage); macrophage colony-stimulating factor 1; M CSF; MCSF; MGC31930
• Gene ID: 1435
• mRNA Refseq: NM_000757
• Protein Refseq: NP_000748
• MIM: 120420
• Uniprot ID: P09603
• Chromosome Location: 1p21-p13
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Cytokines and Inflammatory Response, organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem
• Function: cytokine activity; growth factor activity; growth factor activity; macrophage colony-stimulating factor receptor binding; macrophage colony-stimulating factor receptor binding