Recombinant Mouse CSF1 cell lysate

• Cat.No.: CSF1-001MCL
• Product Overview: Mouse M-CSF / CSF-1 derived in Human Cells. The whole cell lysate is provided in 1X Sample Buffer.

Specification

• Source: Human cells
• Species: Mouse
• Preparation method: Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer with cocktail of protease inhibitors. Cell debris was removed by centrifugation and then centrifuged to clarify the lysate. The cell lysate was boiled for 5 minutes in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 5% b-mercaptoethanol, and lyophilized.
• Lysis buffer: Modified RIPA Lysis Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Quality control Testing: 12.5% SDS-PAGE Stained with Coomassie Blue
• Recommended Usage: 1. Centrifuge the tube for a few seconds and ensure the pellet at the bottom of the tube.2. Re-dissolve the pellet using 200μL pure water and boiled for 2-5 min.3. Store it at -80°C. Recommend to aliquot the cell lysate into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.Notes:The lysate is ready to load on SDS-PAGE for Western blot application. If dissociating conditions are required, add reducing agent prior to heating.
• Stability: Samples are stable for up to twelve months from date of receipt at -80°C
• Storage Buffer: 50 mM Tris-HCl pH 7.4, 150 mM NaCl, 1mM EDTA, 1% Triton X-100, 0.1% SDS, 1% Sodium deoxycholate, 1mM PMSF
• Storage Instruction: Lysate samples are stable for 12 months from date of receipt when stored at -80°C. Avoid repeated freeze-thaw cycles. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes.
• Tag: Non

Gene Information

• Gene Name: Csf1 colony stimulating factor 1 (macrophage) [ Mus musculus ]
• Official Symbol: CSF1
• Synonyms: CSF1; colony stimulating factor 1 (macrophage); macrophage colony-stimulating factor 1; osteopetrosis; op; Csfm; MCSF; C87615
• Gene ID: 12977
• mRNA Refseq: NM_001113529
• Protein Refseq: NP_001107001
• Pathway: Cytokine-cytokine receptor interaction, organism-specific biosystem; Cytokine-cytokine receptor interaction, conserved biosystem; Cytokines and Inflammatory Response (BioCarta), organism-specific biosystem; Hematopoietic cell lineage, organism-specific biosystem; Hematopoietic cell lineage, conserved biosystem; Osteoclast differentiation, organism-specific biosystem; Osteoclast differentiation, conserved biosystem
• Function: cytokine activity; growth factor activity; macrophage colony-stimulating factor receptor binding; protein binding; protein homodimerization activity; protein homodimerization activity

Reviews

10/30/2017

The CSF1 protein is renowned for its exceptional quality and reliability, making it an ideal choice to fulfill my experimental requirements.

03/10/2017

With its high purity and stability, this protein ensures dependable and accurate results, which are crucial for the success of my research endeavors.

06/09/2016

Whether it is for investigating cellular pathways, studying protein functions, or exploring signaling cascades, the CSF1 protein offers unparalleled versatility and performance.

Q&As

How does CSF1 affect cancer progression?

CSF1 is implicated in tumor-associated macrophages, influencing tumor growth, invasion, and the tumor microenvironment.

What are the potential side effects of CSF1 therapy?

Side effects may include immune-related adverse events, inflammation, and alterations in white blood cell counts.

Can CSF1 therapy be combined with other treatments?

Yes, there is ongoing research on combining CSF1-targeted therapies with other immunotherapies or conventional treatments to enhance efficacy.

What are the challenges in developing CSF1-based therapies?

Challenges include maintaining the balance of immune responses, potential off-target effects, and variability in patient responses.

In what ways is CSF1 being researched for neurological disorders?

Studies are exploring its role in neuroinflammation, neuroprotection, and potential therapeutic interventions for conditions like Alzheimer's disease.