Active Recombinant Full Length Human STK17A Protein, N-His-tagged

• Cat.No.: STK17A-65HFL
• Product Overview: N-terminal 6Histagged, recombinant, full length, human DRAK1, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: Protein serine/threonine kinases DRAK1 and DRAK2 (Death Receptor Associated Kinase 1 and 2) have high sequence homology to DAP (Death-associated protein kinase) and ZIP kinases; both DRACKs are exclusively localized to the cell nucleus. Their kinase domains are involved in the apoptotic signaling induced by interferon-γ.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: Full Length
• Form: 2.05mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 89U/mg, where one unit of DRAK1 activity is defined as 1nmol phosphate incorporated into 250μM (KKLNRTLSFAEPG) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 50.4 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MIPLEKPGSG GSSPGATSGS GRAGRGLSGP 61 CRPPPPPQAR GLLTEIRAVV RTEPFQDGYS LCPGRELGRG KFAVVRKCIK KDSGKEFAAK 121 FMRKRRKGQD CRMEIIHEIA VLELAQDNPW VINLHEVYET ASEMILVLEY AAGGEIFDQC 181 VADREEAFKE KDVQRLMRQI LEGVHFLHTR DVVHLDLKPQ NILLTSESPL GDIKIVDFGL 241 SRILKNSEEL REIMGTPEYV APEILSYDPI SMATDMWSIG VLTYVMLTGI SPFLGNDKQE 301 TFLNISQMNL SYSEEEFDVL SESAVDFIRT LLVKKPEDRA TAEECLKHPW LTQSSIQEPS 361 FRMEKALEEA NALQEGHSVP EINSDTDKSE TEESIVTEEL IVVTSYTLGQ CRQSEKEKME 421 QKAISKRFKF EEPLLQEIPG EFIY
• Purity: Purity 82.5% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -20 centigrade. Unopened reagent is stable for a minimum of 6 months from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: STK17A serine/threonine kinase 17a [ Homo sapiens (human) ]
• Official Symbol: STK17A
• Synonyms: STK17A; serine/threonine kinase 17a; DRAK1
• Gene ID: 9263
• mRNA Refseq: NM_004760.3
• Protein Refseq: NP_004751.2
• MIM: 604726
• UniProt ID: Q9UEE5