Active Recombinant Human AKT2(S474D)(120-481) Mutation Protein, N-His-tagged

• Cat.No.: AKT2-16H
• Product Overview: N-terminal 6Histagged recombinant human PKB beta residues 120–end containing the S474D mutation. Expressed by baculovirus in Sf21 insect cells.

Specification

• Description: Akt2, also known as Protein Kinase B Beta, is a putative oncogene encoding a protein belonging to a subfamily of serine/threonine kinases containing SH2-like (Src homology 2-like) domains, which is involved in signaling pathways. Akt2 serves as an oncogene in the tumorigenesis of cancer cells For example, its overexpression contributes to the malignant phenotype of a subset of human ductal pancreatic cancers. The encoded protein is a general protein kinase capable of phosphorylating several known proteins, and has also been implicated in insulin signaling. EMBL M95936. The recombinant protein contains an amino acid substitution, S474D, with reference to the native sequence. This mimics the phosphorylation of S474.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 120-481 aa
• Form: 0.662mg/ml of enzyme in 50mM Tris/HCl pH7.5, 270mM sucrose, 150mM NaCl, 1mM EGTA, 1mM benzamidine, 0.2mM PMSF, 0.1 % 2-mercaptoethanol, 0.03 % Brij-35. Frozen solution.
• Bio-activity: 276U/mg, where one unit of PKB beta activity is defined as 1nmol phosphate incorporated into 30μM modified crosstide (GRPRTSSFAEGKK) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 42.8 kDa
• AA Sequence: 1 MHHHHHHMDY KCGSPSDSST TEEMEVAVSK ARAKVTMNDF DYLKLLGKGT FGKVILVREK 61 ATGRYYAMKI LRKEVIIAKD EVAHTVTESR VLQNTRHPFL TALKYAFQTH DRLCFVMEYA 121 NGGELFFHLS RERVFTEERA RFYGAEIVSA LEYLHSRDVV YRDIKLENLM LDKDGHIKIT 181 DFGLCKEGIS DGATMKTFCG TPEYLAPEVL EDNDYGRAVD WWGLGVVMYE MMCGRLPFYN 241 QDHERLFELI LMEEIRFPRT LSPEAKSLLA GLLKKDPKQR LGGGPSDAKE VMEHRFFLSI 301 NWQDVVQKKL LPPFKPQVTS EVDTRYFDDE FTAQSITITP PDRYDSLGLL ELDQRTHFPQ 361 FDYSASIRE
• Purity: Purity 81.1% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: AKT2 AKT serine/threonine kinase 2 [ Homo sapiens (human) ]
• Official Symbol: AKT2
• Synonyms: AKT2; AKT serine/threonine kinase 2; PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA
• Gene ID: 208
• mRNA Refseq: NM_001243027.3
• Protein Refseq: NP_001229956.1
• MIM: 164731
• UniProt ID: P31751