Active Recombinant Human CLK4(128-end) Protein, N-His-tagged

• Cat.No.: CLK4-51H
• Product Overview: N-terminal 6His-tagged, recombinant, human CLK4 amino acids 128- end expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: CLK4 protein belongs to the CDC2-like protein kinase (CLK) family. This protein kinase can interact with and phosphorylate the serine- and arginine-rich (SR) proteins, which are known to play an important role in the formation of spliceosomes, and thus may be involved in the regulation of alternative splicing. Studies in the Israeli sand rat Psammomys obesus suggested that the ubiquitin-like 5 (UBL5/BEACON), a highly conserved ubiquitin-like protein, may interact with and regulate the activity of this kinase. Multiple alternatively spliced transcript variants have been observed, but the full length natures of which have not yet been determined.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 128-end
• Form: 0.704mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 289U/mg, where one unit of CLK4, active activity is defined as 1nmol phosphate incorporated into 200μM (YRRAAVPPSPSLSRHSSPHQS(p)EDEEE) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 46 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF SKSHRRKRSR SIEDDEEGHL ICQSGDVLRA 61 RYEIVDTLGE GAFGKVVECI DHGMDGMHVA VKIVKNVGRY REAARSEIQV LEHLNSTDPN 121 SVFRCVQMLE WFDHHGHVCI VFELLGLSTY DFIKENSFLP FQIDHIRQMA YQICQSINFL 181 HHNKLTHTDL KPENILFVKS DYVVKYNSKM KRDERTLKNT DIKVVDFGSA TYDDEHHSTL 241 VSTRHYRAPE VILALGWSQP CDVWSIGCIL IEYYLGFTVF QTHDSKEHLA MMERILGPIP 301 QHMIQKTRKR KYFHHNQLDW DEHSSAGRYV RRRCKPLKEF MLCHDEEHEK LFDLVRRMLE 361 YDPTQRITLD EALQHPFFDL LKKK
• Purity: Purity 87.3% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -20 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: CLK4 CDC like kinase 4 [ Homo sapiens (human) ]
• Official Symbol: CLK4
• Synonyms: CLK4; CDC like kinase 4
• Gene ID: 57396
• mRNA Refseq: NM_020666.3
• Protein Refseq: NP_065717.1
• MIM: 607969
• UniProt ID: Q9HAZ1