Active Recombinant Human EPHA1(568-end) Protein, N-His-tagged

• Cat.No.: EPHA1-65H
• Product Overview: N-terminal 6Histagged, recombinant, human EphA1, amino acids 568–end, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: Eph family tyrosine kinase receptors consist of two groups: EphA group members respond to ephrin-A, which is anchored to the cell membrane by glycosylphosphatidylinositol, whereas EphB group members respond to ephrin-B, containing a transmembrane domain. Recently, the Eph-ephrin system has been found to be involved in vascular development, angiogenesis, tumor growth and metastasis as well as regulation of smooth muscle contractility, onset of platelet aggregation and to promote onset, growth and stability of the hemostatic plugs.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 568-end
• Form: 1.39mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 48U/mg, where one unit of EphA1, active activity is defined as 1nmol phosphate incorporated into 0.1mg/ml poly(Glu, Tyr) (4:1) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 50.6 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF RSRRAQRQRQ QRQRDRATDV DREDKLWLKP 61 YVDLQAYEDP AQGALDFTRE LDPAWLMVDT VIGEGEFGEV YRGTLRLPSQ DCKTVAIKTL 121 KDTSPGGQWW NFLREATIMG QFSHPHILHL EGVVTKRKPI MIITEFMENG ALDAFLRERE 181 DQLVPGQLVA MLQGIASGMN YLSNHNYVHR DLAARNILVN QNLCCKVSDF GLTRLLDDFD 241 GTYETQGGKI PIRWTAPEAI AHRIFTTASD VWSFGIVMWE VLSFGDKPYG EMSNQEVMKS 301 IEDGYRLPPP VDCPAPLYEL MKNCWAYDRA RRPHFQKLQA HLEQLLANPH SLRTIANFDP 361 RVTLRLPSLS GSDGIPYRTV SEWLESIRMK RYILHFHSAG LDTMECVLEL TAEDLTQMGI 421 TLPGHQKRIL CSIQGFKD
• Purity: Purity 87% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: EPHA1 EPH receptor A1 [ Homo sapiens (human) ]
• Official Symbol: EPHA1
• Synonyms: EPHA1; EPH receptor A1; EPH; EPHT; EPHT1
• Gene ID: 2041
• mRNA Refseq: NM_005232.5
• Protein Refseq: NP_005223.4
• MIM: 179610
• UniProt ID: P21709