Epha4

  • Official Full Name

    EPH receptor A4

  • Overview

    This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically h
  • Synonyms

    EPHA4; EPH receptor A4; EphA4 , TYRO1; ephrin type-A receptor 4; Hek8; EK8; EPH-like kinase 8; TYRO1 protein tyrosine kinase; tyrosine-protein kinase TYRO1; tyrosine-protein kinase receptor SEK; receptor protein-tyrosine kinase HEK8; SEK; TYRO1;

  • Recombinant Proteins
  • Cell & Tissue Lysates
  • Protein Pre-coupled Magnetic Beads
  • Chicken
  • Cynomolgus Monkey
  • Human
  • Human
  • Mouse
  • Rat
  • Rhesus Macaque
  • E.coli
  • HEK293
  • Human Cell
  • In Vitro Cell Free System
  • Insect Cell
  • Insect Cells
  • Mammalian Cell
  • Mammalian cells
  • Sf21
  • Sf9 Insect Cell
  • Wheat Germ
  • C
  • Fc
  • His
  • hFc
  • DDK|His
  • GST
  • GST|His
  • His (Fc)
  • Avi
  • His|GST
  • N/A
  • N
  • No tag
Species Cat.# Product name Source (Host) Tag Protein Length Price
Human EPHA4-550H Active Recombinant Human EPHA4 Protein, Fc Chimera Mammalian cells Fc
Human EPHA4-68H Active Recombinant Human EPHA4(601-892) Protein, N-His-tagged Sf21 His 601-892 aa
Human EPHA4-366H Recombinant Human EPHA4 Protein, DDK/His-tagged HEK293 DDK/His Val20-Thr547
Human EPHA4-3204H Active Recombinant Human EPHA4 protein(Met1-Thr547), His-tagged HEK293 C-His Met1-Thr547
Human EPHA4-302H Recombinant Human EPH Receptor A4, GST-tagged, Active Sf9 Insect Cell GST
Human EPHA4-3385H Active Recombinant Human EPHA4 Protein, GST/His-tagged Insect Cell GST/His
Human EPHA4-1137H Recombinant Human EPHA4 protein, His & GST-tagged E.coli His/GST Ile621~Ile882 (Accession # P54764)
Human EPHA4-942H Active Recombinant Human EPHA4 protein(Met1-Thr547), His&hFc-tagged HEK293 C-His&hFc Met1-Thr547
Human EPHA4-3384H Active Recombinant Human EPHA4 Protein, GST-tagged Insect Cell GST
Human EPHA4-7315H Recombinant Human EPHA4 protein(Ser570-Val986), His&GST-tagged Insect Cells N-His&GST Ser570-Val986
Human EPHA4-3386H Recombinant Human EPHA4 Protein, GST-tagged Wheat Germ GST
Human EPHA4-26439TH Recombinant Human EPHA4 N/A
Human EPHA4-001HCL Recombinant Human EPHA4 cell lysate Insect Cell N/A
Human EPHA4-1933HCL Recombinant Human EPHA4 cell lysate Human Cell N/A
Human EPHA4-3636H Recombinant Human EPHA4 protein, GST-tagged E.coli GST 904-986 aa
Human EPHA4-4345HF Recombinant Full Length Human EPHA4 Protein, GST-tagged In Vitro Cell Free System GST 986 amino acids
Human EPHA4-0977H Recombinant Human EPHA4 Protein (E252-V986), Tag Free Insect Cell No tag E252-V986
Human EPHA4-3267H Recombinant Human EPHA4 Protein (Val20-Thr547), C-His tagged Mammalian cells C-His Val20-Thr547
Human EPHA4-3087H Recombinant Human EPHA4 protein, His-tagged E.coli His 904-986 aa
Human EPHA4-0978H Recombinant Human EPHA4 Protein (E252-V986), GST tagged Insect Cell GST E252-V986
Human EphA4-1402H Active Recombinant Human EphA4 protein, His-tagged HEK293 His Val20-Thr547
Human EPHA4-3268H Recombinant Human EPHA4 Protein (Val20-Thr547), C-Fc tagged Mammalian cells C-Fc Val20-Thr547
Human EPHA4-67H Recombinant Human EPHA4 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Human EPHA4-67H-B Recombinant Human EPHA4 Protein Pre-coupled Magnetic Beads HEK293
Human EPHA411531H Recombinant Human EPHA4 (606-896) Protein
Human EPHA4-3269H Recombinant Human EPHA4 Protein (Ile621-Ile882), N-GST tagged E.coli N-GST Ile621-Ile882
Mouse Epha4-912M Active Recombinant Mouse EphA4 protein, hFc-tagged HEK293 hFc Met1-Thr547
Mouse Epha4-1724M Recombinant Mouse Eph Receptor A4 Mammalian cells N/A
Mouse Epha4-913M Active Recombinant Mouse Epha4 protein, His-tagged HEK293 His Met1-Thr547
Mouse EPHA4-2136MCL Recombinant Mouse EPHA4 cell lysate Human Cell N/A
Mouse Epha4-10543M-B Recombinant Mouse Epha4 Protein Pre-coupled Magnetic Beads HEK293
Mouse Epha4-10543M Recombinant Mouse Epha4 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Rat Epha4-7440R Active Recombinant Rat Epha4 protein(Met1-Thr547), His-tagged HEK293 C-His Met1-Thr547
Rat Epha4-7439R Active Recombinant Rat Epha4 protein, hFc-tagged HEK293 hFc Met1-Tyr547
Rat EPHA4-1446RCL Recombinant Rat EPHA4 cell lysate Human Cell N/A
Cynomolgus Monkey EPHA4-1192CCL Recombinant Cynomolgus EPHA4 cell lysate Human Cell N/A
Rhesus Macaque EPHA4-1175R Active Recombinant Rhesus EPHA4 Protein, Fc-tagged HEK293 Fc
Rhesus Macaque EPHA4-1176R Active Recombinant Rhesus EPHA4 Protein, His-tagged HEK293 His
Rhesus Macaque EPHA4-1484R Recombinant Rhesus monkey EPHA4 Protein, His-tagged Mammalian Cell His
Rhesus Macaque EPHA4-1309R-B Recombinant Rhesus Macaque EPHA4 Protein Pre-coupled Magnetic Beads HEK293
Rhesus Macaque EPHA4-1309R Recombinant Rhesus Macaque EPHA4 Protein, His (Fc)-Avi-tagged HEK293 His (Fc)-Avi
Chicken EPHA4-6345C Recombinant Chicken EPHA4 Mammalian Cell His
  • Background
  • Quality Guarantee
  • Case Study
  • Involved Pathway
  • Protein Function
  • Interacting Protein
  • Other Resource

What is EPHA4 protein?

EPHA4 (EPH receptor A4) gene is a protein coding gene which situated on the long arm of chromosome 2 at locus 2q36. This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. The EPHA4 protein is consisted of 986 amino acids and its molecular mass is approximately 109.9 kDa.

What is the function of EPHA4 protein?

Upon activation by ephrin ligands, EPHA4 modulates cell morphology and integrin-dependent cell adhesion through regulation of the Rac, Rap and Rho GTPases activity. It plays an important role in the development of the nervous system controlling different steps of axonal guidance including the establishment of the corticospinal projections. In the nervous system, EPHA4 also plays a role in repair after injury preventing axonal regeneration and in angiogenesis playing a role in central nervous system vascular formation. Additionally, its promiscuity makes it available to participate in a variety of cell-cell signaling regulating for instance the development of the thymic epithelium.

EPHA4 Related Signaling Pathway

EPHA4 is involved in many important signaling pathways, which play an important role in cell growth, differentiation, apoptosis, and migration. For example, Ephrin-Eph signaling pathway, ERK signaling pathway, Wnt/β-catenin signaling pathway and so on.

EPHA4 Related Diseases

Due to its important role in signaling pathways, functional abnormalities associated with EPHA4 may lead to a variety of diseases, including neurological developmental disorders (such as congenital brain tumor-like malformations or epilepsy), white matter damage, neurodegenerative diseases (such as Parkinson's disease and Alzheimer's disease), tumors, and more. In addition, mutations in EPHA4 have been linked to a number of human genetic diseases, such as congenital muscular dystrophy and loss of consciousness syndrome.

EPHA4-7.jpg

Fig1. The vicious cycle (surrounded by blue dotted line) was regulated by EphA4-mediated IGF1 synthesis pathway. (Xuefeng Jing, 2016)

Bioapplications of EPHA4

As for the application of EPHA4, although there is currently no clear therapeutic or drug target for EPHA4 treatment, the latest research suggests that EPHA4 may be a potential therapeutic target with the potential to treat neurological diseases and cancer. At the same time, EPHA4 can also be used as a diagnostic indicator to assess the risk and progression of the disease.

High Purity

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EPHA4-2.jpg

Fig2. Activity Data. (EPHA4-3385H)

Case Study 1: Shinichiro Kina, 2023

EPHA4-3.jpg

Fig1. Western blots of OSCC for EphA4 expression and β-actin as a housekeeping control.

Case Study 2: Shinichiro Kina, 2023

EPHA4-4.jpg

Fig2. Differences in the expression of EphA4 in well- and poorly differentiated oral squamous

cell carcinoma (OSCC).

EPHA4-6.jpg

Fig2. llustration of the signaling pathways induced in the progeny of colorectal cancer. Unirradiated (left) and irradiated (right) cells. (Priscila Guimarães de Marcondes, 2017)

Epha4 involved in several pathways and played different roles in them. We selected most pathways Epha4 participated on our site, such as Axon guidance, which may be useful for your reference. Also, other proteins which involved in the same pathway with Epha4 were listed below. Creative BioMart supplied nearly all the proteins listed, you can search them on our site.

Pathway Name Pathway Related Protein
Axon guidanceUNC5A;EZRA;EPHA4A;SCN3B;FGFR1B;EFNA4;RASGRP1;SEMA4D;SLIT2

Epha4 has several biochemical functions, for example, ATP binding, DH domain binding, GPI-linked ephrin receptor activity. Some of the functions are cooperated with other proteins, some of the functions could acted by Epha4 itself. We selected most functions Epha4 had, and list some proteins which have the same functions with Epha4. You can find most of the proteins on our site.

Function Related Protein
ATP bindingATP1A4;EPHA8;VRK2;TAP1;MAPK1;TOR1L3;SMARCAD1;MAPK12;MARS
DH domain binding
GPI-linked ephrin receptor activityEPHA8;EK1;EPHA5;EPHA4;EPHA7;EPHA3
PH domain bindingINPP5A;EPB41L2;EPHA4
ephrin receptor bindingANKS1B;EFNB3;EFNB1;EFNA1;EFNB2;Anks1;SIPA1L1;CRK;EFNB3B
identical protein bindingMPZL2B;ESR1;PAICS;RUNX1T1;DCTPP1;MAP3K13;ERN1;TNK2;ST13
protein bindingMCM10;PARP1;FAM82A2;TMEM115;HPRT1;IFNGR1;DDX58;UBE2R2;SLC4A1
protein kinase activityDYRK1A;SGK3;PKMYT1;VSIG8B;LRRK2;MAP3K1;TBK1;BUB1B;EPHA1
transmembrane-ephrin receptor activityEPHA4;EFNA4;EFNB3;EPHB2;EPHA5;EPHA10;EPHA1;EFNA3;EPHB1

Epha4 has direct interactions with proteins and molecules. Those interactions were detected by several methods such as yeast two hybrid, co-IP, pull-down and so on. We selected proteins and molecules interacted with Epha4 here. Most of them are supplied by our site. Hope this information will be useful for your research of Epha4.

IKBKG; HSP90AB1

Research Area

Related articles

Shi, M; Movius, J; et al. Cerebrospinal Fluid Peptides as Potential Parkinson Disease Biomarkers: A Staged Pipeline for Discovery and Validation. MOLECULAR & CELLULAR PROTEOMICS 14:544-555(2015).
Kempf, A; Montani, L; et al. Upregulation of axon guidance molecules in the adult central nervous system of Nogo-A knockout mice restricts neuronal growth and regeneration. EUROPEAN JOURNAL OF NEUROSCIENCE 38:3567-3579(2013).
  • Q&As
  • Reviews

Q&As (7)

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How does EPHA4 contribute to physiological processes and what is its specific role? 09/21/2021

EPHA4 is a member of the ephrin receptor family of protein kinases that plays an important role in neural development and synaptic plasticity. It is involved in axon guidance, neuronal migration, and cell proliferation.

What roles does the protein EPHA4 play in neuronal development? 01/19/2020

EPHA4 plays a critical role in neuronal development as a member of the Eph receptor family, which are receptor tyrosine kinases involved in various cellular processes. Specifically, EPHA4 regulates axon growth and guidance, acting as a repulsive cue to guide axons in the developing nervous system. Its involvement extends to synaptic plasticity and neuronal survival. Dysregulation of EPHA4 has been linked to neurological disorders, including Alzheimer's disease and neuropathic pain. Further research on the precise mechanisms and downstream signaling pathways of EPHA4 will deepen our understanding of its role in neuronal development and potential therapeutic applications.

How is EPHA4 expression regulated during development? 07/20/2019

EPHA4 expression is tightly regulated during nervous system development, with its expression gradually increasing during embryonic development. Additionally, EPHA4 expression is spatially regulated, with high levels in specific regions of the nervous system. Regulation of EPHA4 expression is mediated by several mechanisms, including transcriptional and post-transcriptional mechanisms.

How is EPHA4 involved in synaptic plasticity? 04/03/2018

EPHA4 is essential for normal synaptic plasticity, with its activation leading to the modulation of various synaptic proteins, such as AMPA receptors, NMDA receptors, and EphB receptors. Dysregulation of EPHA4-mediated signaling has been implicated in multiple neurodegenerative disorders, affecting both synaptic plasticity and axon growth.

What are the signaling mechanisms activated by EPHA4? 03/31/2018

EPHA4 signaling is initiated by binding to its ligands, which leads to activation of downstream signaling pathways. Several signaling pathways are activated by EPHA4, including the MAP kinase pathway, the PI3K-Akt pathway, and the RhoA-ROCK pathway, which play critical roles in the regulation of actin cytoskeleton dynamics.

Can EPHA4 be targeted therapeutically to treat neurodegenerative diseases? 04/07/2017

EPHA4 has emerged as a promising therapeutic target for various neurodegenerative diseases, and several pharmacological inhibitors have been developed to target EPHA4 signaling. These inhibitors have shown efficacy in preclinical models of several neurodegenerative diseases, suggesting their potential in clinical applications.

What is the role of genetic variants in the EPHA4 gene in neurodegenerative diseases? 09/29/2016

There is growing evidence that genetic variants in the EPHA4 gene may contribute to the susceptibility to neurodegenerative diseases, such as Alzheimer's and Parkinson's disease. These variants have been shown to modify EPHA4 expression and function, and may contribute to disease pathogenesis via several mechanisms, including synaptic dysfunction and axon degeneration.

Customer Reviews (3)

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Reviews
03/11/2020

    The results interpretation process with this protein reagent is straightforward, making it suitable for beginners as well.

    10/25/2019

      The composition ratio of the reagent is highly accurate, ensuring the reliability of the experimental results.

      06/10/2016

        Astounding in both reliability and steadfastness, it confers irrefutable persuasion to the experiment.

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