Description : |
Eph (erythropoietin-producing hepatocellular) family tyrosine kinase receptors consist of two groups: EphA group members respond to ephrin-A, which is anchored to the cell membrane by glycosylphosphatidylinositol, whereas EphB group members respond to ephrin-B, containing a transmembrane domain. Among them, only EphA4 can cross-respond to both ephrin-A and -B. EphA5 (EHK1, HEK7, CEK7, TYRO4) is widely distributed in CNS. The localization of EphA5 to motor and sensory neurons is consistent with a role of EphA5 in neural plasticity, cell-cell recognition, and topographical orientation of neuronal systems. |
Source : |
Sf21 |
Species : |
Human |
Tag : |
His |
Protein length : |
655-956 aa |
Form : |
1.32mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
Bio-activity : |
141U/mg, where one unit of EphA5, active activity is defined as 1nmol phosphate incorporated into 0.1mg/ml poly(Glu, Tyr) (4:1) per minute at 30 centigrade with a final ATP concentration of 100μM. |
Molecular Mass : |
37.9 kDa |
AA Sequence : |
1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF TYEDPNQAVH EFAKEIEASC ITIERVIGAG 61 EFGEVCSGRL KLPGKRELPV AIKTLKVGYT EKQRRDFLGE ASIMGQFDHP NIIHLEGVVT 121 KSKPVMIVTE YMENGSLDTF LKKNDGQFTV IQLVGMLRGI SAGMKYLSDM GYVHRDLAAR 181 NILINSNLVC KVSDFGLSRV LEDDPEAAYT TRGGKIPIRW TAPEAIAFRK FTSASDVWSY 241 GIVMWEVVSY GERPYWEMTN QDVIKAVEEG YRLPSPMDCP AALYQLMLDC WQKERNSRPK 301 FDEIVNMLDK LIRNPSSLKT LVNASCRVSN LL |
Purity : |
Purity 89.3% by SDS-PAGE and Coomassie blue staining. |
Applications : |
Drug Discovery & Development |
Storage : |
6 months at -70 centigrade. |
Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |