| Description : |
Eph (erythropoietin-producing hepatocellular) family tyrosine kinase receptors consist of two groups: EphA group members respond to ephrin-A, which is anchored to the cell membrane by glycosylphosphatidylinositol, whereas EphB group members respond to ephrin-B, containing a transmembrane domain. Among them, only EphA4 can cross-respond to both ephrin-A and -B. The expression of EphA8 receptor significantly promoted cell attachment to fibronectin without altering cellular integrin levels. Moreover, p110 phosphatidylinositol (PI) 3-kinase plays as a key bridging molecule between EphA8 and integrins. Inhibition. of EphA8 may disrupt establishment, maintenance, and remodeling of patterns of cellular organization and may also be used in the treatment of various cancers. |
| Source : |
Sf21 |
| Species : |
Human |
| Tag : |
His |
| Protein length : |
615-911 aa |
| Form : |
1.2mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. |
| Bio-activity : |
152U/mg, where one unit of EphA8, active activity is defined as 1nmol phosphate incorporated into 100μM PDKtide per minute at 30 centigrade with a final ATP concentration of 100μM. |
| Molecular Mass : |
37.1 kDa |
| AA Sequence : |
1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF TYEEPGRAGR SFTREIEASR IHIEKIIGSG 61 DSGEVCYGRL RVPGQRDVPV AIKALKAGYT ERQRRDFLSE ASIMGQFDHP NIIRLEGVVT 121 RGRLAMIVTE YMENGSLDTF LRTHDGQFTI MQLVGMLRGV GAGMRYLSDL GYVHRDLAAR 181 NVLVDSNLVC KVSDFGLSRV LEDDPDAAYT TTGGKIPIRW TAPEAIAFRT FSSASDVWSF 241 GVVMWEVLAY GERPYWNMTN RDVISSVEEG YRLPAPMGCP HALHQLMLDC WHKDRAQRPR 301 FSQIVSVLDA LIRSPESLRA TATVSRC |
| Purity : |
Purity 84% by SDS-PAGE and Coomassie blue staining. |
| Applications : |
Drug Discovery & Development |
| Storage : |
6 months at -70 centigrade. |
| Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
| Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |
