Active Recombinant Human JAK3(781-end) Protein, N-His-tagged

• Cat.No.: JAK3-117H
• Product Overview: N-terminal 6Histagged, recombinant, human JAK3 amino acids 781–end, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+ - NTA agarose.

Specification

• Description: Janus kinase 3 (JAK3) is a non-receptor tyrosine kinase belonging to the Janus family. It is activated by a variety of cytokine and growth factor receptors, resulting in signaling cascades that facilitate the activation of various downstream target genes. JAK3 mediates gene transcription through its well-characterized downstream signaling molecules, the signal transducers and activators of transcription proteins (STAT). Inhibition of JAK3 may impair erythropoietin (EPO)-induced erythropoiesis and protection from bain damage following hypoxia. Inhibition may also adversely effect diabetes.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 781-end
• Form: 0.473mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 20mM β-glycerophosphate, 0.25mM sodium orthovanadate, 10mM NaF, 0.1mM EGTA, 0.03% Brij-35, 10% glycerol, 1mM benzamidine, 0.2mM PMSF, 0.1% 2- mercaptoethanol. Frozen solution.
• Bio-activity: 1218U/mg, where one unit of JAK3, active activity is defined as 1nmol phosphate incorporated into 500μM (GGEEEEYFELVKKKK) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 39.9 kDa
• AA Sequence: 1 MHHHHHHISS DYELLSDPTP GALAPRDGLW NGAQLYACQD PTIFEERHLK YISQLGKGNF 61 GSVELCRYDP LGDNTGALVA VKQLQHSGPD QQRDFQREIQ ILKALHSDFI VKYRGVSYGP 121 GRQSLRLVME YLPSGCLRDF LQRHRARLDA SRLLLYSSQI CKGMEYLGSR RCVHRDLAAR 181 NILVESEAHV KIADFGLAKL LPLDKDYYVV REPGQSPIFW YAPESLSDNI FSRQSDVWSF 241 GVVLYELFTY CDKSCSPSAE FLRMMGCERD VPALCRLLEL LEEGQRLPAP PACPAEVHEL 301 MKLCWAPSPQ DRPSFSALGP QLDMLWSGSR GCETHAFTAH PEGKHHSLSF S
• Purity: Purity 44% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: JAK3 Janus kinase 3 [ Homo sapiens (human) ]
• Official Symbol: JAK3
• Synonyms: JAK3; Janus kinase 3; JAKL; LJAK; JAK-3; L-JAK; JAK3_HUMAN
• Gene ID: 3718
• mRNA Refseq: NM_000215.4
• Protein Refseq: NP_000206.2
• MIM: 600173
• UniProt ID: P52333