JAK3 Activity Assay Kit
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Cat.No. : | Kit-1608 Optional Service: Optional requirements on this protein |
Product Overview : | CTK Activity cell lines are engineered to the catalytic domain from CTK of interest fused to the cytokine receptor binding domain (CRBD) of a JAK kinase. The cells co-an Enzyme Acceptor (EA) tagged SH2 domain and a ProLink (PK) tagged Cytokine receptor. The CRBD is designed to bind the CTK catalytic domain to the receptor-PK. Activation of the cytokine receptor-PK induces receptor phosphorylation by the CTK catalytic domain. This leads to SH2-EA recruitment, forcing complementation of the two β-galactosidase enzyme fragments (EA and PK). The resulting functional enzyme hydrolyzes substrate to generate a chemiluminescent signal. These cells have been modified to prevent long term propagation and expansion using a proprietary compound that has no apparent effect on assay performance. |
Size : | 100 dp(1 x 96-well)/200 dp(2 x 96-well)/1000 dp(10 x 96-well) |
Storage : | Short term (2 weeks): Store in vapor phase of liquid N2 |
◆ Recombinant Protein
JAK3-006H | Recombinant Human JAK3 Protein, MYC/DDK-tagged, C13 and N15-labeled | +Inquiry |
JAK3-252H | Recombinant Human JAK3 Protein, His-tagged | +Inquiry |
Jak3-253M | Recombinant Mouse Jak3 Protein, His-tagged | +Inquiry |
JAK3-1672M | Recombinant Mouse JAK3 Protein (818-1100 aa), His-tagged | +Inquiry |
JAK3-4669M | Recombinant Mouse JAK3 Protein, His (Fc)-Avi-tagged | +Inquiry |
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◆ Lysates
JAK3-5106HCL | Recombinant Human JAK3 293 Cell Lysate | +Inquiry |
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