| Cat.No. : |
JAK3-1672M |
| Product Overview : |
Recombinant Mouse JAK3 Protein (818-1100 aa) is produced by Yeast expression system. This protein is fused with a 6xHis tag at the N-terminal. Protein Description: Partial. |
| Description : |
Non-receptor tyrosine kinase involved in various processes such as cell growth, development, or differentiation. Mediates essential signaling events in both innate and adaptive immunity and plays a crucial role in hatopoiesis during T-cells development. In the cytoplasm, plays a pivotal role in signal transduction via its association with type I receptors sharing the common subunit gamma such as IL2R, IL4R, IL7R, IL9R, IL15R and IL21R. Following ligand binding to cell surface receptors, phosphorylates specific tyrosine residues on the Cytoplasmic domain tails of the receptor, creating docking sites for STATs proteins. Subsequently, phosphorylates the STATs proteins once they are recruited to the receptor. Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, upon IL2R activation by IL2, JAK1 and JAK3 molecules bind to IL2R beta (IL2RB) and gamma chain (IL2RG) subunits inducing the tyrosine phosphorylation of both receptor subunits on their Cytoplasmic domain. Then, STAT5A AND STAT5B are recruited, phosphorylated and activated by JAK1 and JAK3. Once activated, dimerized STAT5 translocates to the nucleus and promotes the transcription of specific target genes in a cytokine-specific fashion. |
| Source : |
Yeast |
| Species : |
Mouse |
| Tag : |
His |
| Form : |
Tris-based buffer,50% glycerol |
| Molecular Mass : |
34.2 kDa |
| Protein length : |
818-1100 aa |
| AA Sequence : |
LKYISLLGKG NFGSVELCRY DPLGDNTGPL VAVKQLQHSG PDQQRDFQRE IQILKALHSD FIVKYRGVSY GPGRQSLRLV MEYLPSGCLR DFLQRHRARL HTDRLLLFAW QICKGMEYLG ARRCVHRDLA ARNILVESEA HVKIADFGLA KLLPLGKDYY VVREPGQSPI FWYAPESLSD NIFSRQSDVW SFGVVLYELF TYCDKSCSPS AEFLRMMGPE REGPPLCRLL ELLAEGRRLP PPPTCPTEVQ ELMQLCWAPS PHDRPAFGTL SPQLDALWRG RPG |
| Purity : |
> 90% as determined by SDS-PAGE. |
| Notes : |
Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week. |
| Storage : |
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade. |
| Concentration : |
A hardcopy of COA with reconstitution instruction is sent along with the products. |
| Figure : |
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.