Active Recombinant Human NEK6(2-end) Protein, N-His-tagged

• Cat.No.: NEK6-173H
• Product Overview: N-terminal 6Histagged, recombinant NEK6 amino acids 2–end, expressed in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: The Aspergillus nidulans 'never in mitosis A' (NIMA) gene encodes a serine/threonine kinase that controls initiation of mitosis. NIMA-related kinases (NEKs) are a group of protein kinases that are homologous to NIMA. Evidence suggests that NEKs perform functions similar to those of NIMA.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 2-end
• Form: 0.322mg/ml of enzyme in 50mM Tris/HCl pH8.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 110U/mg, where one unit of NEK6, active activity is defined as 1nmol phosphate incorporated into 300μM (FLAKSFGSPNRAYKK) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 40.5 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMGIRN SKAYVDELTS AGQPGHMPHG GSSNNLCHTL 61 GPVHPPDPQR HPNTLSFRCS LADFQIEKKI GRGQFSEVYK ATCLLDRKTV ALKKVQIFEM 121 MDAKARQDCV KEIGLLKQLN HPNIIKYLDS FIEDNELNIV LELADAGDLS QMIKYFKKQK 181 RLIPERTVWK YFVQLCSAVE HMHSRRVMHR DIKPANVFIT ATGVVKLGDL GLGRFFSSET 241 TAAHSLVGTP YYMSPERIHE NGYNFKSDIW SLGCLLYEMA ALQSPFYGDK MNLFSLCQKI 301 EQCDYPPLPG EHYSEKLREL VSMCICPDPH QRPDIGYVHQ VAKQMHIWMS ST
• Purity: Purity 92% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: NEK6 NIMA related kinase 6 [ Homo sapiens (human) ]
• Official Symbol: NEK6
• Synonyms: NEK6; NIMA related kinase 6; SID6-1512
• Gene ID: 10783
• mRNA Refseq: NM_001145001.3
• Protein Refseq: NP_001138473.1
• MIM: 604884
• UniProt ID: Q9HC98