Recombinant Human NEK6, Active

• Cat.No.: NEK6-662H

Specification

• Species: Human
• Source: E.coli
• Tag: Non
• Description: NEK6 is a serine/threonine kinase that is a member of the Nek family of protein kinases that share an amino-terminal catalytic domain related to NIMA family. Nek6 is a nuclear and cytoplasmic kinase that is required for mitotic progression of human cells. Nek6 is phosphorylated and activated during M phase of the cell cycle. Inhibition of Nek6 function by either overexpression of an inactive Nek6 mutant or elimination of endogenous Nek6 by siRNA arrests cells in M phase and triggers apoptosis.
• Application: Kinase activity is measured as the molar amount of phosphate incorporated into myelin basic protein, per minute per mg protein at 30°C using a final concentration of 50 μM [32P] ATP.
• Biochem/physiol Actions: Nek6 is a nuclear and cytoplasmic kinase that is required for mitotic progression of human cells. It is phosphorylated and activated during M phase of the cell cycle. Inhibition of Nek6 arrests cells in M phase and triggers apoptosis, suggesting that Nek6 is required for metaphase-anaphase transition.
• Physical Form: Solution in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, and 30% glycerol.
• Assay: ≥80% (SDS-PAGE).
• Form: buffered aqueous glycerol solution.
• Mol Wt: protein apparent mol wt ~58 kDa.
• Storage Temp: −70°C.

Gene Information

• Gene Name: NEK6 NIMA (never in mitosis gene a)-related kinase 6 [ Homo sapiens ]
• Synonyms: NEK6; NIMA (never in mitosis gene a)-related kinase 6; SID6-1512; NIMA-related kinase 6; OTTHUMP00000022097; putative serine-threonine protein kinase; EC 2.7.11.1
• Gene ID: 10783
• mRNA Refseq: NM_001145001
• Protein Refseq: NP_001138473
• MIM: 604043
• UniProt ID: P51955
• Chromosome Location: 9q33.3-q34.11
• Function: ATP binding; magnesium ion binding; nucleotide binding; protein phosphatase binding; protein serine/threonine kinase activity; transferase activity; protein serine/threonine kinase activity; signal transducer activity