Description : |
p21-activated kinases (PAK) are serine/threonine kinases, which consist of the three highly homologous members PAK1 to -3, regulated by the GTPases Rac1 and Cdc42. PAKs are numerous and include morphogenetic regulation, modulation of signaling cascades leading to transcriptional regulation, and regulation of apoptotic pathways. PAK2 (PAK65) can monophosphorylate the myosin II regulatory light chain and induce retraction of permeabilized endothelial cells. PAK2 also can be activated by caspase-cleavage in addition to p21-binding. PAK2 inhibitors may have therapeutic potential in cancer treatment. GenBank NM_U24153. The recombinant protein contains the amino acid substitutions L150F, P225T, V383I and (A339 with respect to this accession number. L150F, P225T and (A339 are reported in GenBank NM_U25975. The residue co ordinates in the native sequence have been given. |
Source : |
E. coli |
Species : |
Human |
Tag : |
His and T7,His |
Protein length : |
3-end |
Form : |
4.339mg/ml of enzyme in 50mM Tris/HCl pH 7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 0.2mM PMSF, 1mM benzamidine, 0.1% 2-mercaptoethanol. Frozen solution. |
Bio-activity : |
3216U/mg, where one unit of PAK2 activity is defined as 1nmol phosphate incorporated into 30μM Long S6 peptide per minute at 30 centigrade with a final ATP concentration of 100μM. |
Molecular Mass : |
62.8 kDa |
AA Sequence : |
1 MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSMLDNGE LEDKPPAPPV RMSSTIFSTG 61 GKDPLSANHS LKPLPSVPEE KKPRHKIISI FSGTEKGSKK KEKERPEISP PSDFEHTIHV 121 GFDAVTGEFT GMPEQWARLL QTSNITKLEQ KKNPQAVLDV LKFYDSNTVK QKYLSFTPPE 181 KDGFPSGTPA LNAKGTEAPA VVTEEEDDDE ETAPPVIAPR PDHTKSIYTR SVIDPVPAPV 241 GDSHVDGAAK SLDKQKKKTK MTDEEIMEKL RTIVSIGDPK KKYTRYEKIG QGASGTVFTA 301 TDVALGQEVA IKQINLQKQP KKELIINEIL VMKELKNPNI VNFLDSYLVG DELFVVMEYL 361 AGGSLTDVVT ETCMDEAQIA AVCRECLQAL EFLHANQVIH RDIKSDNVLL GMEGSIKLTD 421 FGFCAQITPE QSKRSTMVGT PYWMAPEVVT RKAYGPKVDI WSLGIMAIEM VEGEPPYLNE 481 NPLRALYLIA TNGTPELQNP EKLSPIFRDF LNRCLEMDVE KRGSAKELLQ HPFLKLAKPL 541 SSLTPLIMAA KEAMKSNRLE HHHHHH |
Purity : |
Purity 71.6% by SDS-PAGE and Coomassie blue staining. |
Applications : |
Drug Discovery & Development |
Storage : |
1 year at -70 centigrade. |
Handling Advice : |
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade. |
Use/Stability : |
On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap. |