Active Recombinant Human STK10(1-348) Protein, N-His-tagged

• Cat.No.: STK10-126H
• Product Overview: N-terminal 6Histagged, recombinant human LOK amino acids 1–348, expressed by baculovirus in Sf21 insect cells. Purified using Ni2+/NTA agarose.

Specification

• Description: LOK, also known as STK10 (Serine/Threonine Kinase 10), a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like Kinase kinases. LOK can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. LOK can also negatively regulate interleukin 2 expression in T-cells via the mitogen activated protein Kinase kinase 1 pathway.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 1-348 aa
• Form: 0.847mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 20mM β-glycerophosphate, 0.25mM sodium orthovanadate, 10mM NaF 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 885U/mg, where one unit of LOK, active activity is defined as 1nmol phosphate incorporated into 250μM (KKSRGDYMTMQIG) per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 43.2 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MAFANFRRIL RLSTFEKRKS REYEHVRRDL 61 DPNEVWEIVG ELGDGAFGKV YKAKNKETGA LVAAKVIETK SEEELEDYIV EIEILATCDH 121 PYIVKLLGAY YHDGKLWIMI EFCPGGAVDA IMLELDRGLT EPQIQEVCRQ MLEALNFLHS 181 KRIIHRDLKA GNVLMTLEGD IRLADFGVSA KNLKTLQKRD SFIGTPYWMA PEVVMCETMK 241 DTPYDYKADI WSLGITLIEM AQIEPPHHEL NPMRVLLKIA KSDPPTLLTP SKWSVEFRDF 301 LKIALDKNPE TRPSAAQLLE HPFVSSITSN KALRELVAEA KAEVMEGIED GRDEGEEEDA 361 VDAASTLENH TQNSSEVS
• Purity: Purity 67.7% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 6 months at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: STK10 serine/threonine kinase 10 [ Homo sapiens (human) ]
• Official Symbol: STK10
• Synonyms: STK10; serine/threonine kinase 10; LOK; PRO2729
• Gene ID: 6793
• mRNA Refseq: NM_005990.4
• Protein Refseq: NP_005981.3
• MIM: 603919
• UniProt ID: O94804