Active Recombinant Human STK38L(83-end) Protein, N-GST,C-His-tagged

• Cat.No.: STK38L-170H
• Product Overview: N-terminal GST-tagged and Cterminal 6His-tagged, recombinant, human NDR2 amino acids 83-end expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography.

Specification

• Description: NDR2 is a member of the N-myc downregulated gene family which belongs to the alpha/beta hydrolase superfamily. The protein encoded by this gene is a cytoplasmic protein that may play a role in neurite outgrowth. This gene may be involved in glioblastoma carcinogenesis. Alternative splicing results in multiple transcript variants.
• Source: Sf21
• Species: Human
• Tag: GST,His
• Protein length: 83-end
• Form: 3.12 mg/ml of enzyme in 50 mM Tris/HCl pH 7.5, 180 mM NaCl, 260 mM Imidazole, 0.09 mM EGTA, 270 mM sucrose, 0.9 mM benzamidine, 0.2 mM PMSF, 0.09% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 18 U/mg, where one unit of NDR2 activity is defined as 1 nmol phosphate incorporated into 300 μM RSRSRSRSRSRSRSR per minute at 30 centigrade with a final ATP concentration of 100 μM.
• Molecular Mass: 72 kDa
• AA Sequence: 1 MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID 61 GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV 121 DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK 181 KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LEVLFQGPEF TRLGLDDFES 241 LKVIGRGAFG EVRLVQKKDT GHIYAMKILR KSDMLEKEQV AHIRAERDIL VEADGAWVVK 301 MFYSFQDKRN LYLIMEFLPG GDMMTLLMKK DTLTEEETQF YISETVLAID AIHQLGFIHR 361 DIKPDNLLLD AKGHVKLSDF GLCTGLKKAH RTEFYRNLTH NPPSDFSFQN MNSKRKAETW 421 KKNRRQLAYS TVGTPDYIAP EVFMQTGYNK LCDWWSLGVI MYEMLIGYPP FCSETPQETY 481 RKVMNWKETL VFPPEVPISE KAKDLILRFC IDSENRIGNS GVEEIKGHPF FEGVDWEHIR 541 ERPAAIPIEI KSIDDTSNFD DFPESDILQP VPNTTEPDYK SKDWVFLNYT YKRFEGLTQR 601 GSIPTYMKAG KLHHHHHH
• Purity: Purity 96% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: STK38L serine/threonine kinase 38 like [ Homo sapiens (human) ]
• Official Symbol: STK38L
• Synonyms: STK38L; serine/threonine kinase 38 like; NDR2
• Gene ID: 23012
• mRNA Refseq: NM_015000.4
• Protein Refseq: NP_055815.1
• MIM: 615836
• UniProt ID: Q9Y2H1