Active Recombinant Human ULK1(1-314) Protein, N-His-tagged

• Cat.No.: ULK1-254H
• Product Overview: N-terminal 6His-tagged, recombinant, human ULK1 amino acids 1-314 expressed by baculovirus in Sf21 insect cells. Purified using immobilized metal affinity chromatography followed by gel filtration.

Specification

• Description: Serine/threonine-protein kinase ULK1 involved in autophagy in response to starvation. It acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. It also acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. Plays a role early in neuronal differentiation and is required for granule cell axon formation.
• Source: Sf21
• Species: Human
• Tag: His
• Protein length: 1-314 aa
• Form: 0.45mg/ml of enzyme in 50mM Tris/HCl pH7.5, 300mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 270mM sucrose, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution.
• Bio-activity: 2858U/mg, where one unit of ULK1 activity is defined as 1nmol phosphate incorporated into 2mg/ml casein per minute at 30 centigrade with a final ATP concentration of 100μM.
• Molecular Mass: 39 kDa
• AA Sequence: 1 MSYYHHHHHH DYDIPTTENL YFQGAMDPEF MEPGRGGTET VGKFEFSRKD LIGHGAFAVV 61 FKGRHREKHD LEVAVKCINK KNLAKSQTLL GKEIKILKEL KHENIVALYD FQEMANSVYL 121 VMEYCNGGDL ADYLHAMRTL SEDTIRLFLQ QIAGAMRLLH SKGIIHRDLK PQNILLSNPA 181 GRRANPNSIR VKIADFGFAR YLQSNMMAAT LCGSPMYMAP EVIMSQHYDG KADLWSIGTI 241 VYQCLTGKAP FQASSPQDLR LFYEKNKTLV PTIPRETSAP LRQLLLALLQ RNHKDRMDFD 301 EFFHHPFLDA SPSVRKSPPV PVPSYPSSGS GSSSSSSSTS HLAS
• Purity: Purity 83% by SDS-PAGE and Coomassie blue staining.
• Applications: Drug Discovery & Development
• Storage: 1 year at -70 centigrade.
• Handling Advice: Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into prechilled micro-centrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 centigrade.
• Use/Stability: On receipt of material store at -70 centigrade. Unopened reagent is stable for a minimum of 1 year from date of shipment when stored at recommended storage temperature. Avoid repeat freeze/thaw cycles. For maximum recovery of product, centrifuge original vial prior to removing the cap.

Gene Information

• Gene Name: ULK1 unc-51 like autophagy activating kinase 1 [ Homo sapiens (human) ]
• Official Symbol: ULK1
• Synonyms: ULK1; unc-51 like autophagy activating kinase 1; ATG1; ATG1A; UNC51; hATG1; Unc51.1
• Gene ID: 8408
• mRNA Refseq: NM_003565.4
• Protein Refseq: NP_003556.2
• MIM: 603168
• UniProt ID: O75385

Reviews

08/06/2022

Reliable results, will reorder.

06/07/2021

Affordable and high-quality.

09/30/2019

Met all my expectations.

Q&As

How does ULK1 interact with other autophagy-related proteins?

ULK1 interacts with several autophagy-related proteins, including ATG13, FIP200, and ATG101, forming a complex that is essential for the initiation of autophagy.

Are there any known upstream regulators of ULK1 activity?

mTOR (mechanistic target of rapamycin) is a known upstream regulator of ULK1. When nutrients are abundant, mTOR is active and inhibits ULK1. Conversely, under nutrient starvation, mTOR is inactivated, leading to ULK1 activation and initiation of autophagy.

What is the primary function of the ULK1 protein in cellular processes?

ULK1 is a serine/threonine-protein kinase that plays a crucial role in the initiation of autophagy, a cellular process responsible for the degradation and recycling of cellular components.

What are the implications of ULK1 dysregulation in diseases like cancer?

Dysregulation of ULK1 can lead to impaired autophagy, which has been implicated in several diseases, including cancer. Overactive or underactive ULK1 can disrupt cellular homeostasis and contribute to tumorigenesis.

Are there any known post-translational modifications of ULK1 that influence its activity?

ULK1 undergoes several post-translational modifications, including phosphorylation and ubiquitination, which can either activate or inhibit its kinase activity and, consequently, its role in autophagy.

How does ULK1 play a role in the initiation of autophagy?

ULK1 is part of a complex that is essential for the initiation of autophagy. Upon activation, ULK1 phosphorylates several targets, leading to the nucleation and formation of the autophagosome.

How does ULK1 activity relate to nutrient availability in cells?

ULK1 activity is sensitive to nutrient availability. Under nutrient-rich conditions, ULK1 is inhibited, while under nutrient-deprived conditions, ULK1 becomes activated, promoting autophagy to recycle cellular components and generate energy.