Active Recombinant Human ULK1 protein, GST&Strep II-Tagged

• Cat.No.: ULK1-10H
• Product Overview: Recombinant Human ULK1 protein(O75385)(M1-A280), fused with N-terminal GST tag and C-terminal Strep II Tag, was expressed in Sf9 Insect Cells.

Specification

• Source: Sf9 Insect Cells
• Species: Human
• Tag: N-GST&C-Strep II
• Protein length: M1-A280
• Form: 50 mM Tris, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
• Bio-activity: The ULK1 activity was detected using Ultra Luminescence technology. The reaction was performed by incubating the ULK1 protein, ATP and substrate at 25°C for 60 min, then adding ADP-Glo reagent and reading RLU signal with BMG.
• Molecular Mass: 59.7 kDa
• Purity: > 95% by SDS-PAGE
• Storage: Stable for 12 months upon stored at -80°C from the date of receipt. And avoid repeated freeze-thaws cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: ULK1 unc-51-like kinase 1 (C. elegans) [ Homo sapiens ]
• Official Symbol: ULK1
• Synonyms: ULK1; unc-51-like kinase 1 (C. elegans); unc 51 (C. elegans) like kinase 1; serine/threonine-protein kinase ULK1; ATG1; ATG1 autophagy related 1 homolog (S. cerevisiae); ATG1A
• Gene ID: 8408
• mRNA Refseq: NM_003565
• Protein Refseq: NP_003556
• MIM: 603168
• UniProt ID: O75385