Recombinant Mouse Ulk1 Protein, Myc/DDK-tagged

• Cat.No.: Ulk1-6830M
• Product Overview: Purified recombinant protein of mouse full-length unc-51 like kinase 1 (Ulk1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy. Phosphorylates FLCN, promoting autophagy.
• Molecular Mass: 112.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ulk1 unc-51 like kinase 1 [ Mus musculus (house mouse) ]
• Official Symbol: Ulk1
• Synonyms: ULK1; Unc-51 like kinase 1 (C. elegans); serine/threonine-protein kinase ULK1; unc-51-like kinase 1; serine/threonine-protein kinase Unc51.1; Unc51.1; AU041434; mKIAA0722
• Gene ID: 22241
• mRNA Refseq: NM_009469
• Protein Refseq: NP_033495
• UniProt ID: O70405