|Cat. No. :
|Product Overview :
||Recombinant human LOK was expressed by baculovirus inSf9 insect cellsusing an N-terminal His tag. MW=43.2 kDa.
||LOK is a member of the Ste20 family of serine/threonine protein kinases, and is similar to several known polo-like kinase kinases. LOK can associate with and phosphorylate polo-like kinase 1, and overexpression of a kinase-dead version of the protein interferes with normal cell cycle progression. LOK can also negatively regulate interleukin 2 expression in T-cells via the mitogen activated protein kinase kinase 1 pathway. MEKK1 and LOK have opposing roles in regulating the enhancer element of the IL-2 gene during T-cell activation. While MEKK1 is responsible for the CD28 signalling pathway that activates the CD28 response element, co-expression of LOK attenuates these effects.
||Sf9 insect cells.
||Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.
|Formulated In :
||25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol, and 1 mM DTT.
||>6 months at –80°C.